N-氨甲酰谷氨酸对断奶环江香猪肠道微生物区系的影响

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (2): 263-267.

N-氨甲酰谷氨酸对断奶环江香猪肠道微生物区系的影响

周笑犁1,2，连国琦1，孔祥峰1,3*，王升平1，印遇龙1,2

1 中国科学院亚热带农业生态研究所，中国科学院亚热带农业生态过程重点实验室，湖南省畜禽健康养殖工程技术研究中心，长沙 410125；2 南昌大学生命科学与食品工程学院，食品科学与技术国家重点实验室，南昌 330047；3 中国科学院环江喀斯特农业生态试验站，环江 547100

出版日期:2014-02-28 发布日期:2014-12-12

Effects of N-carbamyglutamate on Intestinal Microflora of Weaned Huanjiang Mini-pigs

ZHOU Xiao-li1,2, LIAN Guo-qi1, KONG Xiang-feng1,3*, WANG Sheng-ping1, YIN Yu-long1,2

1 Key Laboratory of Agro-ecological Processes in Subtropical Region and Hunan Engineering and Research Center of Animal and Poultry Science,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;2 State Key Laboratory of Food Science and Technology,College of Life Science and Food Engineering,Nanchang University,Nanchang 330047,China; 3 Huanjiang Observation and Research Station for Karst Ecosystems,Chinese Academy of Sciences,Huanjiang 547100,Guangxi

Online:2014-02-28 Published:2014-12-12

摘要/Abstract

摘要： 研究N-氨甲酰谷氨酸（NCG）对断奶仔猪肠道微生物区系的影响。试验选用体重为3.17±0.21 kg的21日龄断奶环江香猪12头，随机分为2组，每组6头，分别饲喂添加0.1% NCG日粮和基础日粮，试验期为14 d。试验结束时，采集回肠和盲肠内容物，利用T-RFLP、PCR/DGGE和荧光定量PCR技术，测定其肠道微生物区系的变化。结果表明，与对照组相比，NCG组回肠内容物中微生物的多样性增加，回肠和盲肠内容物中双歧杆菌和乳酸杆菌的数量显著增加、大肠埃希菌的数量显著降低（P <0.05），盲肠内容物中链球菌的数量也显著降低（P<0.05）。可见，日粮中添加0.1% NCG有助于提高断奶环江香猪肠道内容物中有益菌的数量、抑制有害菌的繁殖，从而改善肠道微生物区系。

关键词: N-氨甲酰谷氨酸, 环江香猪, 肠道微生物区系

Abstract: This study was conducted to investigate the effects of N-carbamoylglutamate(NCG) on intestinal microflora of weaned mini-pigs.Twelve Huanjiang mini-pigs(3.17±0.21 kg of average body weight;weaned at 21 days of age) were assigned randomly into two groups with six replicates in each group.The pigs were fed with a basal diet or 0.1% NCG-supplemented diet for 14 days.At the end of the trial,the contents of ileum and cecum were collected to determine the intestinal microflora by T-RFLP,PCR/DGGE and RT-qPCR technique.The results showed that diet supplemented with NCG increased(P<0.05) the intestinal microflora diversity in ileum,and population of Bifidobacterium and Lactobacillus in ileum and cecum,while decreased(P<0.05) Escherichia in ileum and cecum and Streptococcus in cecum when compared with the control group.These findings suggested that diet supplemented with NCG enriched beneficial intestinal microflora,but suppressed bacterial pathogens in Huanjiang mini-pigs,which effectively promoted the diversity of intestinal microflora.

Key words: N-carbamoylglutamate, Huanjiang mini-pigs, intestinal flora

中图分类号:

R284.2

周笑犁1,2，连国琦1，孔祥峰1,3*，王升平1，印遇龙1,2. N-氨甲酰谷氨酸对断奶环江香猪肠道微生物区系的影响[J]. 天然产物研究与开发, 2014, 26(2): 263-267.

ZHOU Xiao-li1,2, LIAN Guo-qi1, KONG Xiang-feng1,3*, WANG Sheng-ping1, YIN Yu-long1,2. Effects of N-carbamyglutamate on Intestinal Microflora of Weaned Huanjiang Mini-pigs[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(2): 263-267.

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