黄麻链霉菌NF0919菌株发酵液有效成分薄层色谱分离的展开剂优化

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (3): 370-373.

黄麻链霉菌NF0919菌株发酵液有效成分薄层色谱分离的展开剂优化

张文文,杨敬辉,陈露,陈宏州,庄义庆*

江苏丘陵地区镇江农业科学研究所,句容212400

出版日期:2014-03-29 发布日期:2014-12-08

Optimization of TLC Solvent System for the Separation of Chemical Components of Streptomyces corchorusii Strain NF0919 Fermentation Broth

ZHANG Wen-wen, YANG Jing-hui, CHEN Lu, CHEN Hong-zhou, ZHUANG Yi-qing*

Zhenjiang Institute of Agricultural Science,Jurong 212400,China

Online:2014-03-29 Published:2014-12-08

摘要/Abstract

摘要： 根据单因素试验法确定了黄麻链霉菌（Streptomyces corchorusii）NF0919菌株发酵产物的薄层色谱展开剂组分,利用均匀设计法和逐步回归法建立回归模型优化展开剂的配比,最终得出最优化的展开剂系统为氯仿一甲醇一水一冰醋酸= 10:2.75:0.5:0.2,分离效果较理想。

关键词: 展开剂系统优化, 黄麻链霉菌, 薄层色谱

Abstract: A strain of Streptomyces corchorusii (coded as NF0919) was isolated in our laboratory.It was antagonistic to Rhizoctonia solani Kühn and produced alkyl glycoside which had antifungal activity.In this study,the TLC solvent system was developed to separate the chemical components of fermentation broth of Streptomyces corchorusii strain NF0919 based on single factor tests.The TLC solvent system was further optimized using the uniform design and quadratic polynomial stepwise regression.The optimal solvent system was determined to be:chloroform-methanol-water-glacial acetic acid=10:2.75:0.5:0.2.Under the optimal solvent system,the fermentation broth separated clearly.

Key words: optimization of solvent system, Streptomyces corchorusii, TLC

中图分类号:

R284.1

张文文,杨敬辉,陈露,陈宏州,庄义庆*. 黄麻链霉菌NF0919菌株发酵液有效成分薄层色谱分离的展开剂优化[J]. 天然产物研究与开发, 2014, 26(3): 370-373.

ZHANG Wen-wen, YANG Jing-hui, CHEN Lu, CHEN Hong-zhou, ZHUANG Yi-qing*. Optimization of TLC Solvent System for the Separation of Chemical Components of Streptomyces corchorusii Strain NF0919 Fermentation Broth[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(3): 370-373.

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