天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (4): 499-503.

• 研究论文 • 上一篇    下一篇

地榆鞣质对环磷酰胺致小鼠骨髓抑制的保护作用研究

熊永爱1,邹俊波1,杨明1,2*   

  1. 1 成都中医药大学药学院,成都611137;2 江西中医学院中药制剂教育部重点实验室,南昌330004
  • 出版日期:2014-04-30 发布日期:2014-11-19

Protective Effect of Tannins from Sanguisorba officinalis on Cyclophosphamide Induced Myelosuppression in Mice

XIONG Yong-ai1, ZOU Jun-bo1, YANG Min1,2*   

  1. 1 Pharmaceutical College,Chengdu University of Traditional Chinese Medicine,Chengdu 611173, China;2 Jiangxi University of Traditional Chinese Medicine,Key Lab.of Modern Preparation of Traditional Chinese Medicine,Ministry of Education,Nanchang 330004, China
  • Online:2014-04-30 Published:2014-11-19

摘要: 为了观察地榆鞣质对骨髓抑制的保护作用,本文采用腹腔注射环磷酰胺致小鼠骨髓抑制模型。造模成功后,将骨髓抑制小鼠按体重随机分为:模型组、集落刺激因子组、地榆鞣质20、10、5 mg/kg三个剂量组,另设正常对照组。连续灌胃10 d,每天一次,以外周血液白细胞数量、骨髓细胞DNA含量和CD34+的表达以及骨髓细胞O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)蛋白的表达为评价指标,研究地榆鞣质对CTX所致骨髓抑制的治疗作用。结果显示地榆鞣质可显著升高小鼠外周血WBC(P<0.05);显著升高小鼠骨髓DNA含量(P<0.05);显著促进CD34+的表达(P<0.05);显著促进MGMT蛋白的表达(P<0.05)。通过本研究可以看出地榆鞣质可显著拮抗CTX所致小鼠骨髓抑制。

关键词: 地榆鞣质, 环磷酰胺, 骨髓抑制, 拮抗

Abstract: The objective of this study was to investigate the protective effect of tannins from Sanguisorba officinalis (Great Burnet) on myelosuppression in mice.The myelosuppression model was induced by peritoneal injection of cyclophosphamide (CTX).Then the myelosuppression mice were randomly divided into:model group,colony-stimulating factor group,three Great Burnet tannins treatment groups of different dosage (20,10,5 mg/kg),and normal group.The drugs were chronically administrated to myelosuppression mice once a day for 21 days.The counts of peripheral blood leukocytes,DNA contents and CD34+ expression of bone marrow cells and the O6-methyl guanine-DNA methyltransferase (MGMT) protein expression were used as main indexes and were determined.The results showed that Great Burnet tannins significantly increased peripheral WBC (P<0.05);significantly elevated bone marrow DNA content (P<0.05);significantly promoted the expression of CD34+ (P<0.05);also significantly promoted MGMT protein expression (P<0.05).Based on these results,it was concluded that Great Burnet tannins can significantly antagonize CTX induced myelosuppression in mice.

Key words: Great Burnet tannins, cyclophosphamide, myelosuppression, antagonism

中图分类号: 

R285.5