HPLC-DAD和UPLC-MS/MS对蔓花生中二苯乙烯类化合物的研究

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (5): 699-703.

HPLC-DAD和UPLC-MS/MS对蔓花生中二苯乙烯类化合物的研究

韩寒冰1*，王明阳2，马超1，李海航3

1 广东石油化工学院化学与生命科学学院；2 茂名出入境检验检疫局，茂名525000；3 华南师范大学生命科学学院，广东省植物发育生物工程重点实验室，广州510631

出版日期:2014-05-31 发布日期:2014-11-17

Simultaneous Determination of Stilbene Compounds in Arachis duranensis by HPLC-DAD and UPLC-MS/MS

HAN Han-bing1*, WANG Ming-yang2, MA Chao1, LI Hai-hang3

1 College of Chemistry and Life Sciences,Guangdong University of Petrochemical Technology,Maoming; 2 Maoming Entry-Exit Inspection and Quarantine Bureau,Maoming,Guangdong 525000,China; 3 Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,College of Life Sciences,South China Normal University,Guangzhou 510631, China

Online:2014-05-31 Published:2014-11-17

摘要/Abstract

摘要： 采用HPLC-DAD和UPLC-MS/MS对蔓花生中白藜芦醇、白藜芦醇苷和二苯乙烯苷三种二苯乙烯类化合物进行鉴定，建立HPLC-DAD同时测定三种化合物含量的方法。HPLC-DAD分析采用依利特ODS1色谱柱，柱温40 ℃，流动相A为甲醇，B为1%乙酸水溶液，流速1.0 mL/min。UPLC-MS/MS采用ACQUITY UPLC BEH C₁₈色谱柱，流动相A为乙腈，B为水，电喷雾离子源，负离子检测，数据采集方式为多反应监测模式（MRM）。结果表明：蔓花生根、茎和叶中白藜芦醇含量分别为66.28、90.83和81.56 μg/g，白藜芦醇苷含量分别为123.78，302.77和236.53 μg/g，根和茎中二苯乙烯苷含量分别为673.60和764.65 μg/g，叶中未检测到二苯乙烯苷。

关键词: 蔓花生, 二苯乙烯类化合物, HPLC-DAD, UPLC-MS/MS

Abstract: Simultaneous determination of 3 stilbene compounds,namely resveratrol,polydatin and stilbene glucoside (2,3,5,4′-tetra-hydroxy-stilbene-2-O-β-D-glucoside) from Arachis duranensis was performed using HPLC-DAD and UPLC-MS/MS.The experimental conditions for HPLC-DAD analysis were as follows:ODS1 column (250 mm × 4.6 mm,5 μm);column temperature,40 ℃;mobile phase A,methanol;mobile phase B,1% aqueous acetic acid;flow rate,1.0 mL/min;DAD scanning wavelength,190-400 nm;detection wavelength,303 nm.The UPLC-MS/MS conditions were as follows:ACQUITY UPLC BEH C₁₈ column (2.1 mm × 50 mm,1.7 μm);mobile phase A,acetonitrile;mobile phase B,water;ion source,ESI (negative ion mode).UPLC-MS/MS data were collected using multiple reaction monitoring (MRM) mode.The results showed that resveratrol contents were 66.28,90.83 and 81.56 μg/g,polydatin contents were 123.78,302.77 and 236.53 μg/g,in roots,stems and leaves of A.duranensis,respectively.The content stilbene glucoside in roots and stems was 673.60 and 764.65 μg/g,respectively,but was not detected in leaves.

Key words: Arachis duranensis, stilbene compounds, HPLC-DAD, UPLC-MS/MS

中图分类号:

R286

韩寒冰1*，王明阳2，马超1，李海航3. HPLC-DAD和UPLC-MS/MS对蔓花生中二苯乙烯类化合物的研究[J]. 天然产物研究与开发, 2014, 26(5): 699-703.

HAN Han-bing1*, WANG Ming-yang2, MA Chao1, LI Hai-hang3. Simultaneous Determination of Stilbene Compounds in Arachis duranensis by HPLC-DAD and UPLC-MS/MS[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(5): 699-703.

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