关键词: 旱莲草, 凝血活性, 溶血活性

Abstract: Procoagulant and haemolytic constituents of Eclipta alba were studied,which would provided the basis for the development and utilization of E.alba. Six compounds were isolated from E.alba by various chromatographic techniques.Their chemical structures were identified as eclalbasaponin I (1), eclalbasaponin IV (2), eclalbasaponin V (3 ), 3,4,5-trihydroxy-6-(2-hydroxy-3-(palmitoyloxy)prop-oxy)-etrahydro-2H-pyran-yl) methanesulfonic acid (4), wedelolactone (5) and isodemethylwedelolactone (6) on the basis of the spectroscopic data. Compound 4 was isolated from this plant for the first time.The tests of hemolytic activity in vitro indicated that compound 2,3 and 4 showed strong haemolytic activity,with haemolytic rates of (93.52±1.56)%,(82.55±2.72)% and (94.99±0.68)% respectively at the concentration of 0.6 μg/mL.The tests of procoagulant activity in vitro indicated that compounds 5 and 6 showed strong procoagulant activity.They can directly agglomerate hemocytes or transform fibrinogen into fibrin,which in turn agglomerated the hemocytes.

Key words: Eclipta alba, procoagulant activity, haemolytic activity