荷叶离褶伞高产漆酶菌株选育及其漆酶酶学特性

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (8): 1178-1181.

荷叶离褶伞高产漆酶菌株选育及其漆酶酶学特性

梁倩倩1,2，魏生龙1，丁玲强2，闫红霞2，梁艳萍2，席亚丽1*

1 河西学院食用菌研究所；2 河西学院农业与生物技术学院，张掖 734000

出版日期:2014-08-30 发布日期:2014-11-06

Mutational Screening of Lyophyllum decastes with High Yield of Laccase and Characteristics of Laccase from the Mutant

LIANG Qian-qian1,2, WEI Sheng-long1, DING Ling-qiang2, YAN Hong-xia2, LIANG Yan-ping2, XI Ya-li1*

1 Institute of Edible Fungi,Hexi University; 2 Department of Life Science and Engineering,Hexi University,Zhangye 734000 ,China

Online:2014-08-30 Published:2014-11-06

摘要/Abstract

摘要： 应用原生质体紫外诱变，对一株产漆酶荷叶离褶伞（Lyophyllum decastes）菌株紫外诱变，用愈创木酚马铃薯葡萄糖固体平板变色法初筛,然后用ABTS ［2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐］测定漆酶活性进行复筛,获得1株漆酶高产诱变菌株HY1022-01；对HY1022-01所产漆酶粗酶液酶学特性进行研究，结果表明，HY1022-01所产漆酶最大酶活力比出发菌株提高30.32%，最适条件下酶活达991.67 U/mL，且产酶稳定；HY1022-01所产漆酶最适作用温度为35 ℃，在30~35 ℃较为稳定；最适反应pH值为3.0，pH在2.2~3.8较为稳定，ABTS的漆酶动力学常数为72.622 μM。

关键词: 荷叶离褶伞, 漆酶, 紫外诱变

Abstract: The protoplasts of one Lyophyllum decastes were exposed to UV radiation and the mutants were screened on guaiacol-containing PDA plates.The laccase activities of the mutated strains were further determined by ABTS.A mutant strain HY 1022-01 with high production yield of laccase was obtained.The further investigation on the characteristics of laccase from mutant strain HY 1022-01 showed that the maximum laccase activity increased 30.32% compared to its parent strain and up to 991.67 U/mL in optimum condition.The laccase activity was stable in 30-35 ℃ and pH of 2.2-3.8,while the optimal temperature and pH was 35 ℃ and 3.0 respectively.The Km was 0.72622 μM on the basis of ABTS.

Key words: Lyophyllum decastes, laccase, ultraviolet mutagenesis

中图分类号:

S646.2

梁倩倩1,2，魏生龙1，丁玲强2，闫红霞2，梁艳萍2，席亚丽1*. 荷叶离褶伞高产漆酶菌株选育及其漆酶酶学特性[J]. 天然产物研究与开发, 2014, 26(8): 1178-1181.

LIANG Qian-qian1,2, WEI Sheng-long1, DING Ling-qiang2, YAN Hong-xia2, LIANG Yan-ping2, XI Ya-li1*. Mutational Screening of Lyophyllum decastes with High Yield of Laccase and Characteristics of Laccase from the Mutant[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(8): 1178-1181.

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