广西产黄杞叶不同溶媒提取物中二氢黄酮醇的定量分析

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (8): 1257-1260.

广西产黄杞叶不同溶媒提取物中二氢黄酮醇的定量分析

付晓雪1，宋明明1，周辉2，史丽颖1，唐玲1，王永奇1*

1 大连大学药物研究所，大连116622；2 广西钦州市农业机械学校，钦州 535000

出版日期:2014-08-30 发布日期:2014-11-06

Determination of Total Dihydroflavonols in Extract of Engelhardtia roxburghianan Wall. Leaves

FU Xiao-xue1, SONG Ming-ming1, ZHOU Hui2, SHI Li-ying1, TANG Ling1, WANG Yong-qi1*

1 Institute of Materia Medica of Dalian University,Dalian 116622 ,China;2 Agricultural Machinery Technology College of Qinzhou,Qinzhou 535000 ,China

Online:2014-08-30 Published:2014-11-06

摘要/Abstract

摘要： 为了测定黄杞叶不同溶媒提取物中主要化学成分二氢黄酮醇的含量,以落新妇苷为标准品,采用分光光度法测定黄杞叶醇提物、水提物及醇提物的PE层、EtoAc层、H₂O层和水提物的EtoAc层、H₂O层中总二氢黄酮醇的含量。结果表明：落新妇苷在12.3~28.7 μg/mL范围内呈良好的线性关系（R²=0.9991）；该方法灵敏度高、稳定性好,可用于黄杞叶提取物的质量控制。

关键词: 黄杞叶提取物, 总二氢黄酮醇, 落新妇苷

Abstract: The objective of this study was to determine the content of total dihydroflavonols in different extracts of Engelhardtia roxburghiana wall.leaves.The contents of total dihydroflavonols in ethanol extract and its PE,EtoAc and water fractions as well as water extract and its EtoAc and water fractions of E.roxburghiana leaves were determined by spectrophotometry with astilbin as standard.The results indicated that astilbin showed a good linear relationship in the range of 12.3~28.7μg/mL (R²=0.9991); the developed method was with high sensitivity,good stability,and it is applicable for the quality control of E.roxburghiana leaves.

Key words: Engelhardtia roxburghiana Wall.leaves, total dihydroflavonols, astilbin

中图分类号:

R284

付晓雪1，宋明明1，周辉2，史丽颖1，唐玲1，王永奇1*. 广西产黄杞叶不同溶媒提取物中二氢黄酮醇的定量分析[J]. 天然产物研究与开发, 2014, 26(8): 1257-1260.

FU Xiao-xue1, SONG Ming-ming1, ZHOU Hui2, SHI Li-ying1, TANG Ling1, WANG Yong-qi1*. Determination of Total Dihydroflavonols in Extract of Engelhardtia roxburghianan Wall. Leaves[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(8): 1257-1260.