栀子苷和牛血清白蛋白相互作用研究

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (9): 1375-1379.

栀子苷和牛血清白蛋白相互作用研究

高义霞1*，刘增文2，周向军1，袁毅君1

1 天水师范学院生命科学与化学学院，天水741001；2 和田县第二高级中学，和田848400

出版日期:2014-09-30 发布日期:2014-11-02

The Interaction between Gardenoside and Bovine Serum Albumin

GAO Yi-xia1*, LIU Zeng-wen2, ZHOU Xiang-jun1, YUAN Yi-jun1

1 College of Life Science and Chemistry,Tianshui Normal University,Gansu province,Tianshui 741001, China; 2 No.2 High School of Hetian,Xinjiang Uygur Autonomous Region, Hetian 848400, China

Online:2014-09-30 Published:2014-11-02

摘要/Abstract

摘要： 在模拟生理条件下，利用紫外和荧光光谱法研究栀子苷和牛血清白蛋白相互作用。通过Stern-Volmer方程和Lineweaver-Burk考察栀子苷对牛血清白蛋白内源性荧光的猝灭机制，分别在298 K、310 K和322 K下利用结合常数和结合位点数计算反应体系的热力学参数。结果表明，当温度为298 K、302 K和322 K时，栀子苷对牛血清白蛋白的猝灭常数分别为4.632×10⁴、3.515×10⁴和3.575×10⁴ mol/L，结合常数K_A分别为1.805×10⁴、2.546×10⁴和4.165×10⁴，结合位点数分别为1.334、1.112和0.944，栀子苷对牛血清白蛋白的猝灭方式属于静态猝灭；热力学参数ΔG＜0，ΔH＜0，ΔS＞0，表明栀子苷与牛血清白蛋白结合作用力为静电引力，根据Forster非辐射共振能量转移理论，计算出栀子苷与牛血清白蛋白之间的结合距离为1.78 nm。

关键词: 牛血清白蛋白, 栀子苷, 荧光光谱

Abstract: The interaction between gardenoside and bovine serum albumin (BSA) was studied by fluorescence and UV-Vis absorption spectrometry under simulated physiological conditions. The quenching mechanism of the intrinsic fluorescence of BSA by gardenoside was investigated by Stern-Volmer and Lineweaver-Burk equations.It was proved that the quenching mechanism of BSA by gardenoside was a static quenching procedure with quenching constants of 4.632×10⁴, 3.515×10⁴ and 3.575×10⁴ mol/L at 298, 310 and 322K, respectively. Binding constants and number of binding sites at corresponding temperature were 1.805×10⁴, 2.546×10⁴ and 4.165×10⁴ as well as 1.334, 1.112 and 0.944, respectively. The thermodynamic parameters were ΔG＜0，ΔH＜0，ΔS＞0, which proved the electrostatic force played a major role between BSA and gardenoside.The distance was calculated to be 1.78 nm using Fosters resonance energy transfer.

Key words: Bovine serum albumin, gardenoside, fluorescence spectrometry

中图分类号:

TQ351

高义霞1*，刘增文2，周向军1，袁毅君1. 栀子苷和牛血清白蛋白相互作用研究[J]. 天然产物研究与开发, 2014, 26(9): 1375-1379.

GAO Yi-xia1*, LIU Zeng-wen2, ZHOU Xiang-jun1, YUAN Yi-jun1. The Interaction between Gardenoside and Bovine Serum Albumin[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(9): 1375-1379.

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