天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (9): 1527-1530.

• 开发研究 • 上一篇    下一篇

源于葡糖杆菌属细菌纤维素材料的检测分析

李利军1*,卢美欢1,马英辉1,王银存2   

  1. 1 陕西省微生物研究所,西安 710043;2 西北大学生命科学学院,西安 710069
  • 出版日期:2014-09-30 发布日期:2014-11-02

Detection and Characteristic Analysis of Bacterial Cellulose Produced by Gluconobacter

LI Li-jun1*, LU Mei-huan1, MA Ying-hui1, WANG Yin-cun2   

  1. 1 Shaanxi Province Institute of Microbiology,Xi'an 710043 ,China; 2 College of Life Sciences,Northwest University,Xi'an 710069, China
  • Online:2014-09-30 Published:2014-11-02

摘要: 利用自筛的葡糖杆菌属菌株AXB(X),发酵获得产物细菌纤维素(BC),通过傅立叶红外光谱、X-射线衍射以及扫描电子显微镜等仪器研究其材料特性,确认BC是高纯度的纤维素,且晶型属于纤维素I型;通过酶解和酸解试验显示该产物纯度达95%以上,持水率在95%以上。此外,扫描电镜观察其产物表面形态结构表明纤维相互交织成具有超细精密多孔的网络结构,且具有比滤纸、棉纤维和桑皮纤维更高的结晶度。

关键词: 葡糖杆菌属, 细菌纤维素, 检测, 特性

Abstract: In this study,the bacterial cellulose (BC) was produced by fermenting a Gluconacetobacter strain AXB(X). The material properties were investigated by Fourier-transform infrared spectrometry, X-ray diffraction and scanning electron microscopy. The results proved that BC was high purity cellulose,and the crystal structure of the product belonged to cellulose type I. Enzymolysis and acidolysis experiments showed the purity of BC was over 95%. The water holding capacity was more than 95%. The observation of surface morphology of the product,by scanning electron microscopy,revealed that the superfine,precise and porous network structure of BC was formed by interfelting fibers. The crystallinity of BC was higher than filter paper fiber,cotton fiber and mulberry fiber.

Key words: Gluconobacter, bacterial cellulose, detection, characteristics

中图分类号: 

R318.08