关键词: 大叶小檗, 抗肿瘤活性, H₂₂荷瘤

Abstract: To study the amur barberry root 75% ethanol extract and total alkaloid of H₂₂ mice tumorburdened bone tumor inhibition and provide the basis for further development of its medical value.According to the standard method for vaccination H₂₂ mice tumor-burdened bone cells,randomly divided into 7 groups,respectively given different doses of amur barberry root produced by intraperitoneal injection of 75% ethanol extract,amur barberry root total alkaloid,physiological saline,cyclophosphamide,gather the tumor after 12 d,block,spleen and thymus weight and calculate the tumor inhibition rate,spleen and thymus index are calculated separately,and at the same time.Give medicine group,tumor weight significantly less than the negative control group and drug group compared with negative groups had extremely significant difference(P<0.01),75% ethanol extract and total alkaloids in the concentration of 500 mg/kg YiLiuLv were greater than 60%,when the concentration of 1000 mg/kg,both YiLiuLv are the same,and the effect is best,can reach 73.83%,negative immune organ index and the blank group comparison has the extremely significant difference(P<0.01),amur barberry root 75% ethanol extract and total alkaloids in high and low dose group,the thymus index were higher than in positive group,lower than that of negative group,spleen index were higher than in positive group,75% ethanol extract and total alkaloids in the dose of 1000 mg/kg to influence a tumorburdened mice immune organs,to medicine group index,the blank group and positive control group also had extremely significant difference(P<0.01).The amur barberry root 75% ethanol extract and total alkaloids could better inhibiting H₂₂ tumor-burdened in mice liver cancer in its growth.

Key words: Berberis amurensis, Antitumor activity, H₂₂ tumor-burdened