不同产地和品种浙贝母中贝母素乙含量HPLC分析

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl2): 256-259.

不同产地和品种浙贝母中贝母素乙含量HPLC分析

王忠华*，郭芙，张惠恩，沈秋，庄欣晨

浙江万里学院生物技术研究所,宁波 315100

出版日期:2014-12-30 发布日期:2015-03-05

HPLC Analysis of Peiminine Content of Fritillaria thunbergii of Different Species and from Different Areas

WANG Zhong-hua*,GUO Fu,ZHANG Hui-en,SHEN Qiu,ZHUANG Xin-chen

Institute of Biotechnology,Zhejiang Wanli University,Ningbo 315100,China

Online:2014-12-30 Published:2015-03-05

摘要/Abstract

摘要： 本文以宁波章水、金华磐安、江苏南通3个不同产地和狭叶、宽叶、多籽3个不同品种的浙贝母鳞茎为材料，采用Hypersil C₁₈ ODS2（4.6 mm×150 mm，5 μm)，流动相为CH₃CN（A）和100 mmol/L CH₃COONH₄（pH5.0）（B），流速为1.0 mL/min，进样量为10 μL，检测波长为375 nm，并以贝母素乙为参照峰，初步建立了不同产地和不同品种的浙贝母HPLC特征图谱，并根据峰面积计算出浙贝母主成分生物碱贝母素乙的含量，结果表明不同产地依次为宁波章水、江苏南通和金华磐安，分别为0.323%、0.281%和0.194%；不同品种依次为狭叶、宽叶和多籽，分别为0.317%、0.269%和0.214%。该法建立的HPLC代谢谱可为浙贝母不同产地和品种的鉴别提供参考。

关键词: 浙贝母, 贝母素乙, HPLC分析

Abstract: In this study,the contents of peiminine in bulbs of Fritillaria thunbergii from 3 different areas:Zhangshui region at Ningbo city,Panan county at Jinhua city,Nantong county at Jiangsu provience and 3 different species:narrow leaves,broad leaves and multibulb cultivars were determined using high performance liquid chromatography (HPLC).Chromatographic separation was carried out on an Hypersil C₁₈ column (4.6 mm × 150 mm,5 μm) eluted with CH₃CN (A) and 100 mmol/L CH₃COONH₄ (pH 5.0) (B) at a flow rate of 1.0 mL/min.The detection wavelength was set at 375 nm.The sample volume was 10 μL.The HPLC specific chromatogram of F.thunbergii from different areas and species was primarily constructed and the content of peiminine was gained according to peak areas.The results showed that the contents of peiminine in F.thunbergii from Zhangshui region at Ningbo city,Panan county at Jinhua city,Nantong county at Jiangsu provience were 0.323%,0.281% and 0.194%,respectively.The contents of peiminine in narrow leaves,broad leaves,multi-bulb species of F.thunbergii were 0.317%,0.269% and 0.214%,respectively.HPLC metabolic profile developed by this experiment can be used to identify F.thunbergii from different areas and species.

Key words: Fritillaria thunbergii Miq, peiminine, HPLC analysis

中图分类号:

R284.2

王忠华*，郭芙，张惠恩，沈秋，庄欣晨 . 不同产地和品种浙贝母中贝母素乙含量HPLC分析[J]. 天然产物研究与开发, 2014, 26(Suppl2): 256-259.

WANG Zhong-hua*,GUO Fu,ZHANG Hui-en,SHEN Qiu,ZHUANG Xin-chen. HPLC Analysis of Peiminine Content of Fritillaria thunbergii of Different Species and from Different Areas[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(Suppl2): 256-259.

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