天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl2): 260-263.

• 研究简报 • 上一篇    下一篇

不同产地盘龙七中熊果苷含量的分析研究

王星,赵桦*   

  1. 陕西理工学院生物科学与工程学院,汉中 723000
  • 出版日期:2014-12-30 发布日期:2015-03-05

Determination of Arbutin in Rhizoma Bergeniae scopulosae from Different Areas

WANG Xing,ZHAO Hua*   

  1. College of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723000,China
  • Online:2014-12-30 Published:2015-03-05

摘要: 本文采用高效液相色谱法,测定了14个不同产地盘龙七中熊果苷的含量。色谱条件为:色谱柱为Inertsil ODS3 C18(4.6×150 mm,5 μm)柱,以甲醇:水(8:92)为流动相,流速1.0 mL/min,检测波长282nm,柱温30 ℃。熊果苷在10.3~103.0 μg/mL范围内呈良好的线性关系,Y=4468.8X-999.10 (R2=0.9999),平均加样回收率99.58%,RSD为1.79%(n=6)。研究结果表明,盘龙七中含有较丰富的熊果苷,其含量随产地的不同而显示出一定的差异,其变化范围在18.32 ~5.34 mg/g,甘肃省文县产盘龙七的熊果苷含量最高(18.32 mg/g)。本研究建立的熊果苷含量测定方法简便、准确、重复性好,可用于该药材中熊果苷含量的测定和药材质量控制。

关键词: 盘龙七, 秦岭岩白菜, 熊果苷, 高效液相色谱法

Abstract: The contents of arbutin in Rhizoma Bergeniae Scopulosae from different areas were determined using HPLC.The chromatographic separation was carried out using Inertsil ODS3 C18 column(4.6 mm×150 mm,5 μm),the mobile phase was methanol:water (8:92),the detection wavelength was set at 282 nm,the temperature was 30 ℃,and the flow rate was 1.0 mL/min.The calibration curve was linear in the range of 10.3-103.0 μg/mL and the regression equation for arbutin was Y=4468.8X-999.1 (R2=0.9999),the average recovery was 99.58% with RSD of 1.79%(n=6).The result showed that there are some different in the content of arbutin among different origins,the contents of arbutin were range between 18.32 mg/g to 5.34 mg /g.This method is simple,efficient and accurate,which can be used for the analysis of arbutin in Rhizoma Bergeniae Scopulosae.

Key words: Rhizoma Bergeniae Scopulosae, Bergenia scopulosa T.P.Wang, Arbutin, HPLC

中图分类号: 

R284.1