天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl2): 268-272.

• 开发研究 • 上一篇    下一篇

人参皂苷Rg1键合硅胶固定相的制备、表征及应用(一)

韩亚琼1,2,谷晓娟1,李心怡1,陈喜龙1,王娟1 ,沈报春1*   

  1. 1 昆明医科大学药学院暨云南省天然药物药理重点实验室,云南 昆明 650500; 2 新乡医学院第二附属医院,河南 新乡 453002
  • 出版日期:2014-12-30 发布日期:2015-03-05

Preparation,Characterization and Application of Ginsenoside Rg1-bonded Silica Gel Stationary Phase(I)

HAN Ya-qiong1,2 ,GU Xiao-juan1,LI Xin-yi1,CHEN Xi-long1,WANG Juan1,SHEN Bao-chun1*   

  1. 1 School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University, Kunming Yunnan 650500,China; 2 The Second Affiliated Hospital of Xinxiang Medical University,Xinxiang Henan 453002,China
  • Online:2014-12-30 Published:2015-03-05

摘要: 采用“一锅法”,以1,6-己二异氰酸酯为偶联剂,制备出一种新型天然产物药用成分键合固定相-人参皂苷Rg1键合硅胶固定相。以固体核磁共振等手段对该固定相的结构进行表征。采用该固定相首次建立同时测定血塞通成药中三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1、人参皂苷Rb3和人参皂苷Rd六种皂苷的含量的方法。人参皂苷Rg1键合硅胶固定相同时测定血塞通中六种主要皂苷的含量,有助于其质量控制。以人参皂苷Rg1键合硅胶固定相分离三七中的皂苷成分,为天然产物的分离分析提供新的思路。

关键词: 人参皂苷Rg1键合硅胶固定相, 结构表征, 皂苷, 血塞通, 含量测定

Abstract: A new ginsenoside Rg1-bonded silica gel stationary phase with natural active ingredient was prepared by “one-pot” reaction method,using 1,6-diisocyanate as spacer arm.The characterization of new self-made ginsenoside Rg1-bonded silica gel stationary was achieved by solid state NMR.An HPLC method for determination of six saponins,including notoginsenoside R1,ginsenoside Rg1, ginsenoside Re,ginsenoside Rb1,ginsenoside Rb3 and ginsenoside Rd in Xuesaitong was first developed by HPLC,using ginsenoside Rg1-bonded silica gel stationary phase.Six components of saponins of Panax nototginseng could be simultaneously determined by ginsenoside Rg1-bonded silica gel stationary phase,which will benefit for quality control.It’s a new idea for natural products analysis using ginsenoside Rg1-bonded silica gel stationary phase.

Key words: ginsenoside Rg1-bonded silica gel stationary phase, characterization of stationary phase, determination, saponin , Xuesaitong

中图分类号: 

R282 R917 O658