天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl2): 299-304.

• 开发研究 • 上一篇    下一篇

青霉属真菌P-9生物转化罗汉果总苷的研究

赵丽娟，司鹏鹏，茅仁刚*   

  1. 上海师范大学，上海 200234
  • 出版日期:2014-12-30 发布日期:2015-03-05

Dtermination of Transformation Products in Microbial Transformation of Cucubitane Triterpene Glycosides from Momordica grosvenori by Penicillium sp P-9 by HPLC

ZHAO Li-juan,SI Peng-peng,MAO Ren-gang*   

  1. Shanghai Normal University,Shanghai 200234,China
  • Online:2014-12-30 Published:2015-03-05

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摘要： 采用青霉属真菌P-9对罗汉果总苷进行生物转化，主要转化产物A、B、C经熔点、比旋度、质谱(MS)、红外光谱(IR)和核磁共振(NMR)波谱分析，鉴定为：A为罗汉果醇-24-O-β-D-葡萄吡喃糖基-(1→2)-β-D-葡萄吡喃糖苷；B为罗汉果醇-3-O-β-葡萄吡喃糖基-24-O-β-D-葡萄吡喃糖基-(1→2)-β-D-葡萄吡喃糖苷；C为11-酮-罗汉果醇-3-O-β-D-葡萄吡喃糖基-24-O-β-D-葡萄吡喃糖基-(1→2)-β-D-葡萄吡喃糖苷，A、B、C三种化合物均未见文献报道。采用高效液相色谱法，以C18 ODS为色谱柱，乙腈（A）-水（B）作为流动相进行梯度洗脱，检测波长为：203 nm，柱温：30 ℃，流速：1 mL/min，进样量：10 μL，测得在底物加量为0.5%，28 ℃，220 r/min下摇瓶培养120 h，主要产物A、B、C的含量最高分别为1.006、0.293、0.025 mg/mL。在选定的色谱条件下转化产物A、B线性范围良好，转化产物A、B的平均加样回收率分别为99.3%、97.8%，RSD分别为1.9%、1.2%。

关键词: 高效液相色谱, 微生物转化, 罗汉果总苷, 转化产物A、B、C

Abstract: We found a penicillium sp J-9 which can transform Momordica grosvenori into products A,B and C.Three products were identified by melting point,MS,IR,NMR.The method of HPLC for the transformation products A 、B and C.ZW＆A C18 (250×4.60 mm,5 μm)column was used as the stationry phase.The mobile phase was acetonitrile:water,the UV detection wavelength was 203 nm,the column temperature was 30 ℃,the flow rate was 1.0 mL/min,the injection volume was 10 μL.The transformation last 120 h under 28 ℃，220r/min and substrate at a concertration of 0.5%.The three kinds of products had changeable contents in different time.Results show that the content of products A and B indicates a good linearity under these conditions with average recoveries of （99.3±1.12）% and（97.8±1.68）% and RSDs of 1.9% and 1.2%.

Key words: HPLC, microbial transformation, mogrosides, transformation products A,B and C

中图分类号: 

Q939.9

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