天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (10): 1712-1716.doi: 10.16333/j.1001-6880.2015.10.005

• 研究论文 • 上一篇    下一篇

荷叶黄酮对乳酸杆菌和双歧杆菌抗氧化能力的协同作用研究

刘双环1,李欢1,王聪1,梁博1,胡夏韵1,韩蓓1,2*   

  1. 1 西安交通大学医学部;2 陕西省营养与食品安全工程研究中心,西安 710061
  • 出版日期:2015-10-30 发布日期:2015-10-30

Synergistic Effect of Lotus Leaf Flavonoids on Antioxidant Activity of Lactobacillus bulgaricus and Bifidobacterium animalis

LIU Shuang-huan1, LI Huan1, WANG Cong1, LIANG Bo1, HU Xia-yun1, Han Bei1,2*   

  1. 1 Health Science Center Xian Jiaotong university; 2 Nutrition and Food Safety Engineering Research Center of shaanxi Province,Xian 710061,China
  • Online:2015-10-30 Published:2015-10-30

摘要: 为研究荷叶黄酮对乳酸杆菌和双歧杆菌抗氧化能力的影响,0.25 mg/mL的荷叶黄酮添加至培养基培养保加利亚乳杆菌(Lactobacillus bulgaricus A105)、动物双歧杆菌(Bifidobacterium animalis B211),并提取细胞裂解液,依次从自由基清除能力、抗脂质过氧化能力及抗氧化物质活性三方面评估其抗氧化能力。结果显示,0.25 mg/mL的荷叶黄酮提高了动物双歧杆菌(54.9%)及保加利亚乳杆菌(14.8%)对O2·-清除能力;其对保加利亚乳杆菌清除DPPH自由基能力稍有提升,对动物双歧杆菌清除DPPH自由基能力稍有抑制;其对保加利亚乳杆菌抗脂质过氧化能力提升率达658%,具有显著的协同增效作用。同时发现:经添加0.25 mg/mL的荷叶黄酮至培养基后,动物双歧杆菌和保加利亚乳杆菌胞内超氧化物歧化酶(SOD)酶活及谷胱甘肽(GSH)活力得到显著的提高。结果表明,荷叶黄酮的添加对保加利亚乳酸杆菌和动物双歧杆菌的抗氧化活性具有促进作用,且对保加利亚乳酸杆菌抗脂质过氧化能力具有明显的协同促进作用,可能与其胞内SOD酶活及GSH活力得到的显著的提升有关,具有潜在的应用价值。

关键词: 荷叶黄酮, 益生菌, 抗氧化能力, 协同作用

Abstract: The in vitro antioxidant capacity of Lactobacillus bulgaricus A105 and Bifidobacterium animalis B211 were evaluated by adding 0.25 mg/mL lotus leaf flavonoids into the culture medium during growth.The cell lysis solution was extracted and the antioxidant activities were analyzed through radical scavenging ability and inhibition effect on lipid preoxidation.The results showed that 0.25 mg/mL of lotus leaf flavonoids can improve the O2 scavenging activity of B.animalis (54.9%) and L.bulgaricus (14.8%) in vitro. Also,lotus leaf flavonoids have a slight increasing effect on the DPPH radical ability on L.bulgaricus while a slight inhibition effect on B.animalis. It can significantly improve the anti-lipid peroxidation activity of L.Bulgaricus, the increasing rate reached to 658%,which showed obvious synergistic effect.In addition,it can significantly improve intracellular enzyme activity of superoxide dismutase (SOD) and glutathione (GSH) vitality in B.animalis and L.bulgaricus. In conclusion,lotus leaf flavonoids had synergistic effects on the antioxidant activity of B.animalis and L.bulgaricus. All those results showed the addition of lotus leaf flavonoids had some promoting effects on the antioxidant activities of B.animalis and L.bulgaricus, especially high synergistic effect on anti-lipid peroxidation activity of L.bulgaricus, which was possibly caused by increasing SOD and GSH activities with the addition of lotus leaf flavonoids.

Key words: lotus leaf flavonoid, probiotics, antioxidant capacity, synergistic effect

中图分类号: 

Q939.9