蜜楝花及果实中吴茱萸碱、吴茱萸次碱及内酯含量积累变化研究

doi:10.16333/j.1001-6880.2015.12.014

天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (12): 2069-2073.doi: 10.16333/j.1001-6880.2015.12.014

蜜楝花及果实中吴茱萸碱、吴茱萸次碱及内酯含量积累变化研究

马英丽，杨秋琛，尹阳阳，赵桦*

陕西理工学院生物科学与工程学院，汉中 723000

出版日期:2015-12-29 发布日期:2016-01-06

Accumulation of Evodin,Evodiamin and Rutaecarpine in Flowers and Fruits of Evodia lenticellata Huang

MA Ying-li, YANG Qiu-chen, YIN Yang-yang, ZHAO Hua*

Biological Science and Engineering College,Shaanxi University of Technology,Shaanxi Hanzhong 723000,China

Online:2015-12-29 Published:2016-01-06

摘要/Abstract

摘要： 分析测定了不同生长时期蜜楝花及果实中吴茱萸生物碱及吴茱萸内酯含量及其积累变化。色谱条件为：色谱柱为Inertsil ODS-3 C₁₈柱（4.6×150 mm，5 μm），以乙腈-四氢呋喃-水（41:1:58）为流动相，流速1.0 mL/min，检测波长225 nm，柱温30 ℃。蜜楝中吴茱萸内酯在49.65~248.25 μg/mL范围内呈良好的线性关系，Y=1955.4X-5577.3（R²=0.9996），平均加样回收率102.70%，RSD为3.00%（n=6）；吴茱萸碱在8.88~88.80 μg/mL范围内呈良好的线性关系，Y=89913X+73191（R²=0.9990），平均加样回收率103.99%，RSD为2.18%（n=6），吴茱萸次碱在8.25~49.49 μg/mL范围内呈良好的线性关系，Y=56127X-51856（R² =0.9990），平均加样回收率103.50%，RSD为1.48%（n=6）。随着发育阶段的推移，蜜楝花、果实中吴茱萸碱、吴茱萸次碱及吴茱萸内酯的含量逐渐增加，在近成熟的果实中三种成分含量最高。研究结果表明，蜜楝果实中生物碱和吴茱萸内酯的含量丰富，可作为提取制备吴茱萸生物碱及吴茱萸内酯成分的一种植物资源，具有很好的开发利用价值。

关键词: 蜜楝, 吴茱萸碱, 吴茱萸次碱, 吴茱萸内酯

Abstract: In this study,the accumulation of evodin,evodiamin and rutaecarpine in the flower and fruit of Evodia lenticellata Huang was investigated using high performance liquid chromatography (HPLC).An Inertsil ODS-3 C₁₈ column (4.6×150 mm,5 μm) was used with acetonitrile,tetrahydrofuran and ultra pure water (41:1:58) as mobile phases.The flow rate was 1.0 mL/min.The detection wavelength was 225 nm and column temperature was kept at 30 ℃.Rutaecarpine had good linearity in the range of 49.65-248.25 μg/mL,Y=1955.4X-5577.3 (R²=0.9996).The average recovery was 102.70% with RSD of 3.00% (n=6);Evodin had good linearity in the range of 8.88-88.80 μg/mL,Y=89913X+7319 1(R²=0.9990).The average recovery was 103.99% with RSD of 2.18% (n=6);Evodiamin had good linearity in the range of 8.25-49.49 μg/mL,Y=56127X-51856 (R²=0.9990).The average recovery was 103.50% with RSD of 1.48% (n=6).The results showed that the content of evodin,evodiamin and rutaecarpine increased constantly with the growth of E.lenticellata and reached the highest when the fruits were close to maturity.The fruits of E.lenticellata were rich in evodin,evodiamin and rutaecarpine.

Key words: Evodia lenticellata Huang, evodin, evodiamin, rutaecarpine

中图分类号:

Q949.95

马英丽，杨秋琛，尹阳阳，赵桦*. 蜜楝花及果实中吴茱萸碱、吴茱萸次碱及内酯含量积累变化研究[J]. 天然产物研究与开发, 2015, 27(12): 2069-2073.

MA Ying-li, YANG Qiu-chen, YIN Yang-yang, ZHAO Hua*. Accumulation of Evodin,Evodiamin and Rutaecarpine in Flowers and Fruits of Evodia lenticellata Huang[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(12): 2069-2073.

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蜜楝花及果实中吴茱萸碱、吴茱萸次碱及内酯含量积累变化研究

Accumulation of Evodin,Evodiamin and Rutaecarpine in Flowers and Fruits of Evodia lenticellata Huang

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