中药复方对猪源耐药大肠杆菌分离株的体外抑制作用

doi:10.16333/j.1001-6880.2015.S.029

天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (Suppl): 117-121.doi: 10.16333/j.1001-6880.2015.S.029

中药复方对猪源耐药大肠杆菌分离株的体外抑制作用

杨建英1,2，郭小权3，肖莉春1,3，黄名钱1,3，孔祥峰1*

1 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室，湖南省畜禽健康养殖工程技术研究中心，长沙 410125；2 河南科技大学医学技术与工程学院，洛阳 471003；3 江西农业大学动物科学技术学院，南昌 330045

出版日期:2015-12-01 发布日期:2016-01-12

In vitro Inhibitory Effects of Chinese Herbal Formulae against Antibiotic-resistant Isolates of E.coli from Swine

YANG Jian-ying1,2, GUO Xiao-quan3, XIAO Li-chun1,3, HUANG Ming-qian1,3, KONG Xiang-feng1*

1 Key Laboratory of Agro-ecological Processes in Subtropical Region,Hunan Engineering and Research Center of Animal and Poultry Science,Institute of Su btropical Agriculture,Chinese Academy of Sciences, Changsha 410125,China; 2 College of Medical Technology and Engineering,He′nan University of Science and Technology,Luoyang 471003,China; 3 College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China

Online:2015-12-01 Published:2016-01-12

摘要/Abstract

摘要： 为了筛选对耐药大肠杆菌具有抑制作用的中药复方，为临床大肠杆菌病的防治提供依据，本研究利用前期研究筛选出的体外抑菌作用较强的12味中药，根据配伍原则组成5个复方，制备其水提液，采用打孔法、倍比稀释法测定其对10个猪源耐药大肠杆菌分离株和3个大肠杆菌标准株的体外抑制活性。结果表明，5个复方对同一受试菌的抑菌圈大小依次为：复方5>复方4>复方1（SEC616、SEC911、SEC1284和C197除外）>复方2>复方3。复方5对13株受试菌的抑菌圈直径最大、MIC值最小，提示其体外抑菌作用最强；复方3对13株受试菌的抑菌圈直径最小、MIC值最大，提示其体外抑菌作用最弱；复方1、复方2和复方4对13株受试菌的抑菌圈直径和MIC也存在差异。上述结果提示，复方5对耐药大肠杆菌的体外抑制作用最强，可作为养猪生产中防治猪大肠杆菌病的备选方剂。

关键词: 中药复方, 大肠杆菌, 抑菌作用, 猪

Abstract: This study was conducted to screen out Chinese herbal formula with antimicrobial activity against antibiotic-resistant E.coli, and provide basis for prevention and clinical cure of colibacillosis.According to the principles of compatibility,5 different kinds of Chinese herbal formulae were made by 12 kinds of Chinese herbal medicines with strong antimicrobial activity,screened out from our previous study,and then their in vitro antimicrobial activities against 10 isolated strains of antibiotic-resistant E.coli and 3 type strains of E.coli were evaluated by determining the diameter of inhibition zone (IZD) and minimal inhibitory concentration (MIC).Results showed that different formulae had different IZD on the same tested bacteria,in other words,the IZD were Formula 5 > Formula 4 > Formula 1 (except for SEC616,SEC911,SEC1284,and C197)> Formula 2 > Formula 3.The Formula 5 had the maximum IZD and minimum MIC against the 13 tested strains of E.coli, respectively,which suggested that this formula presented the strongest in vitro antibacterial activity;the Formula 3 had the minimum IZD and maximum MIC against 13 tested strains of E.coli, which suggested that Formula 3 presented the weakest in vitro antibacterial activity.In addition,the IZD and MIC values of Formula 1,Formula 2,and Formula 4 against different tested strains of E.coli were also different.These findings indicated that Formula 5 had the strongest in vitro antimicrobial activity against antibiotic-resistant E.coli, which could be an alternative prescription for prevention and cure of colibacillosis in swine production.

Key words: Chinese herbal formula, E.coli, antimicrobial activity, swine

中图分类号:

S853.74

杨建英1,2，郭小权3，肖莉春1,3，黄名钱1,3，孔祥峰1*. 中药复方对猪源耐药大肠杆菌分离株的体外抑制作用[J]. 天然产物研究与开发, 2015, 27(Suppl): 117-121.

YANG Jian-ying1,2, GUO Xiao-quan3, XIAO Li-chun1,3, HUANG Ming-qian1,3, KONG Xiang-feng1*. In vitro Inhibitory Effects of Chinese Herbal Formulae against Antibiotic-resistant Isolates of E.coli from Swine[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(Suppl): 117-121.

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中药复方对猪源耐药大肠杆菌分离株的体外抑制作用

In vitro Inhibitory Effects of Chinese Herbal Formulae against Antibiotic-resistant Isolates of E.coli from Swine

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