天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (Suppl): 122-127.doi: 10.16333/j.1001-6880.2015.S.030

• 开发研究 • 上一篇    下一篇

甘露聚糖酶低限度水解改善银耳多糖水提液粘度

樊琳娜1,贾焱1,宋丽雅1,王领2,何聪芬1*   

  1. 1 北京工商大学理学院 北京市植物资源研究开发重点实验室,北京 100048;2 北京华夏众芳生物科技有限公司,北京 100036
  • 出版日期:2015-12-01 发布日期:2016-01-12

Reduce the Viscosity of Tremella Polysaccharide Extract with β-Mannase

FAN Lin-na1, JIA Yan1, SONG Li-ya1, WANG Ling2, HE Cong-fen1   

  1. 1 Beijing Key Laboratory of plant resources research and development;School of Science,Beijing Technology and Business University;100048,Beijing,China;2 Beijing Huaxiazhongfang Biotechnology Co.Ltd.100036,Beijing,China
  • Online:2015-12-01 Published:2016-01-12

摘要: 利用甘露聚糖酶对银耳多糖进行低限度水解,降低银耳多糖水提液的粘度及提取物平均分子量,以改善银耳多糖提取液因为粘度过大在工业生产中不易操作的缺陷。确定酶解的最适条件为:料液比 1:100,温度 60 ℃,pH 5.0~6.0,加酶量 0.08%,酶解时间1 h。该条件下得到提取液的粘度约为26 mPa·s,平均分子量由26480降至16530 ,在104以下分布由10%提高至20%,106以下分布由35%提高至50%,此水解为低限度水解。

关键词: 银耳多糖, 甘露聚糖酶, 低限度水解, 粘度, 分子量

Abstract: It has been hard to filter or purify tremella polysaccharide extract in industrial operations because of the high viscosity of the extract.β-Mannase was used for reduce the average molecular weight and then reduce the viscosity of tremella polysaccharide extract in its extraction process.Reacting under the enzyme concentration of 0.08% for 1 h,the hydrolysis got its best result.The average molecular weight was reduced from 26480 to 16530 and the proportion of small molecules increased.With a final viscosity of 26 mPa·s,the hydrolysis did improve the filtration and purification of tremella polysaccharide extract in industrial operations.

Key words: tremella polysaccharide, Mannase, hydrolysis, viscosity, molecular weight

中图分类号: 

TS241