天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (Suppl): 151-155.doi: 10.16333/j.1001-6880.2015.S.036

• 开发研究 • 上一篇    下一篇

响应面法优化冻绿叶中黄酮类物质的提取工艺

高霞1,孔冬梅1*,卫晓军2   

  1. 1 山西大学生命科学学院,太原 030006;2 交口县卫明西医诊所,吕梁 032400
  • 出版日期:2015-12-01 发布日期:2016-01-12

Optimization of Extraction of Flavonoids from the Leaves of Rhamnus utilis by Response Surface Methodology

GAO Xia1,KONG Dong-mei1*,WEI Xiao-jun2   

  1. 1 College of Life Science, Shanxi University,Taiyuan 030006,China; 2 WeiMing Western Medicine Clinic of Jiaokou County,Lvliang 032400,China
  • Online:2015-12-01 Published:2016-01-12

摘要: 利用超声波辅助提取技术,以乙醇溶液为溶剂,采用单因素试验考察乙醇体积分数、液料比、提取时间和提取温度对冻绿叶黄酮提取量的影响,并通过响应面法优化冻绿叶黄酮的提取工艺。得到黄酮类物质的最佳提取条件:乙醇体积分数70%、液料比33:1、提取时间50 min和提取温度30 ℃。在此条件下,黄酮提取量理论值为35.18 mg/g,实测值为35.07 mg/g,与理论值基本吻合。

关键词: 冻绿叶, 黄酮, 提取, 响应面

Abstract: Flavonoids in Rhamnus utilis were extracted with ultrasonic-assisted extraction technology using ethanol as solvent.A series of single-factor experiments were made to investigate the effects of ethanol concentration,liquid/solid ratio,extraction time and temperature on flavonoids yield.Response surface method was used to optimize the extraction process.The optimum extraction parameters were determined as:ethanol concentration of 70%,liquid/solid ratio of 33:1,extraction time of 50 min and temperature of 30 ℃.Under these conditions,the extraction yield of total flavonoids was 35.07 mg/g,which was consistent with the predicted value (35.18 mg/g).

Key words: Rhamnus utilis, flavonoids, extraction, response surface

中图分类号: 

O629