天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (Suppl): 32-38.doi: 10.16333/j.1001-6880.2015.S.010

• 研究论文 • 上一篇    下一篇

3种块菌香气成分的GC-MS分析

吴素蕊,游金坤,张鑫,高观世*   

  1. 中华全国供销合作总社昆明食用菌研究所,昆明 650221
  • 出版日期:2015-12-01 发布日期:2016-01-12

Analysis of Aromatic Compound in Three Kinds of Truffles by GC-MS

WU Su-rui,YOU Jin-kun,ZHANG Xin,GAO Shi-guan*   

  1. Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooporatives,Kunming 650221,China
  • Online:2015-12-01 Published:2016-01-12

摘要: 本文采用同时蒸馏萃取法对印度块菌(Tuber indicum Cooke & Massee)、白块菌(Tuber sp.)、拟凹陷块菌(Tuber pseudoexcavatum Y.Wang)的香气成分分别进行提取,使用气相色谱-质谱联用(GC-MS)法结合计算机检索进行香气成分分析和鉴定,应用峰面积归一法确定各成分的相对含量。结果表明,在印度块菌、白块菌、拟凹陷块菌的香气成分提取物中分别分离出了101个、71个、49个峰;分别鉴定出91种、58种、36种化合物;分别占其总组分的81.09%、81.28%、63.92%。综合比较分析,印度块菌香气成分最为丰富,且比较均衡,白块菌次之,拟凹陷块菌种类较少。但白块菌中丰富的烯醇类香气成分和拟凹陷块菌中较高的辛烯醇含量都使其具有很高的应用价值。

关键词: 块菌, 香气成分, 气相色谱-质谱法, 同时蒸馏萃取

Abstract: Simultaneous distillation extraction method(SDE) was used to extracte the aroma components from Tuber indicum Cooke & Massee, Tuber sp.and Tuber pseudoexcavatum Y.Wang.By means of the GC-MS and computer researching the component of them was analysed and identified.The relative content of each component was determined by area normalization method.This study demonstrates that among the three truffles the aroma components peaks was extracted at the number of 101,71,49 and identified 100,63,41 compounds respectively.Comprehensively comparative analysis shows that Tuber indicum Cooke & Massee aroma components are most abundant and relatively balanced,followed by Tuber sp.,and Tuber pseudoexcavatum Y.Wangs.But the abundant Alcohol-based aroma components in Tuber sp.and the rich Octenol in Tuber pseudoexcavatum Y.Wang make them being of great value.

Key words: Tuber huidongense, aromatic compound, gas chromatography-mass spectrometry, simultaneous distillation extraction method

中图分类号: 

S-3