天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (1): 112-119.doi: 10.16333/j.1001-6880.2016.1.020

• 开发研究 • 上一篇    下一篇

野生和栽培川西獐牙菜醇提物红外光谱分析及药效比较研究

杨红霞1,2，李岑1,2，高婷婷1,2，耿卢婧1,2,3，魏立新1,2，杜玉枝1,2*   

  1. 1 中国科学院西北高原生物研究所 青海省藏药药理学和安全性评价研究重点实验室；2 中国科学院藏药研究重点实验室，西宁 810008；3 中国科学院大学，北京 100049
  • 出版日期:2016-01-29 发布日期:2016-02-01

Infrared Spectroscopy Analysis and Pharmacological Activity of Ethanol Extracts of Wild and Cultivated Swertia mussotii Franch

YANG Hong-xia1,2,LI Cen1,2,GAO Ting-ting1,2,GENG Lu-jing1,2,3,WEI Li-xin1,2, DU Yu-zhi1,2*   

  1. 1 Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Qinghai key laboratory of Tibetan Medicine Pharmacology and Safety Evaluation; 2 Key  Laboratory of Tibetan Medicine Research,Chinese Academy of Sciences,Xining 810008,China; 3University of Chinese Academy of Sciences,Beijing 100049,China
  • Online:2016-01-29 Published:2016-02-01

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摘要： 比较野生和栽培川西獐牙菜醇提物的成分及其肝保护、镇痛、抑制肠蠕动等药理作用。采用HPLC方法，红外光谱分析技术分析两种醇提物的成分；采用CCl4致小鼠肝损伤模型、小鼠扭体实验和小鼠小肠推进功能实验对其进行药效学研究。野生和栽培川西獐牙菜原药材中含獐牙菜苦苷、龙胆苦苷和芒果苷的总量分别为3.949%和4.539%，两种原药材的一维和二维红外光谱间均没有明显差异；两种醇提物的二维红外光谱之间差异较显著，但均有显著的预防肝损伤的作用，明显抑制醋酸所致的炎性疼痛和正常小鼠的肠蠕动功能，二者药效没有明显差异，且栽培品随给药剂量的增加而增强药效的趋势比野生品明显。

关键词: 川西獐牙菜, 野生, 栽培, 红外光谱, 药效

Abstract: To compare the ingredient and pharmacological activity of ethanol extracts of wild and cultivated Swertia mussotii Franch. The method of HPLC and infrared spectroscopy was used to compare the ingredient of extracts of wild and cultivated S. mussotii Franch;liver injury model induced by CCl4 in mice,acetic acidinduced writhing experiment and normal mouse were used to compare the hepatoprotective function,abirritation and peristaltic inhibition of the extracts. The total content of swertiamarin,gentiopicrin and mangiferin of wild and cultivated S. mussotii Franch were 3.949% and 4.539% respectively;Infrared spectroscopy of ethanol extracts had no obvious difference,while two-dimensional correlation spectroscopy (2D-COS) of them had evident difference. The pharmacological experiments results proved their hepatoprotective function,abirritation and peristaltic inhibition,while the enhancement pharmacological activity of cultivated sample with the increasing of dosage was more obvious when it compared with that of wild sample.

Key words: Swertia mussotii Franch, wild, cultivated, infrared spectroscopy, pharmacological activity

中图分类号: 

R965.1

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