天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (11): 1802-1805.doi: 10.16333/j.1001-6880.2016.11.021

• 开发研究 • 上一篇    下一篇

小剂量辣椒素对慢性应激大鼠胃动力的影响及机制研究

王缕,史孝敏,彭燕*   

  1. 西南医科大学附属医院消化内科,泸州 646000
  • 出版日期:2016-11-30 发布日期:2017-03-30

Effect and Mechanism of Low Doses of Capsaicin on Gastric Motility of Rat with Chronic Stress

WANG Lv,SHI Xiao-min,PENG Yan*   

  1. The Affiliated Hospital of Southwest Medical University Gastroenterology,Luzhou 646000,China
  • Online:2016-11-30 Published:2017-03-30

摘要: 通过检测不同小剂量辣椒素(Cap)对慢性应激大鼠模型(CUMS)胃排空率、胃窦和脑组织内辣椒素受体(TRPV1)、神经降压素(NT)的变化,探讨Cap对大鼠胃动力的作用及机制。建立CUMS大鼠诱导胃动力障碍模型,对造模大鼠给予三种不同剂量Cap 0.04、0.4、4 mg/kg处理2 w,采用胃酚红排空率检测各组大鼠胃动力的变化情况,并用ELISA法测定胃窦、脑组织中TRPV1、NT表达。结果显示模型组大鼠胃酚红排空率显著低于正常组,三种剂量Cap干预后CUMS大鼠胃酚红排空率均显著高于模型对照组(P<0.05),且随着Cap剂量的增加,胃酚红排空率反而降低。同时在Cap干预后胃窦及脑组织内TRPV1、NT的表达随着Cap剂量的增加而增加。可见3种小剂量的Cap可改善CUMS大鼠胃排空障碍,以0.04 mg/kg组效果最佳。实验结果表明小剂量Cap可改善CUMS大鼠胃排空障碍,其作用机制可能与胃及脑组织内TRPV1、NT的表达有关。

关键词: 辣椒素, 胃动力, TRPV1, NT

Abstract: To study the effect of Capsaicin (Cap) with different low dosages on the gastric motility,transient receptor potential vanilloid1 (TRPV1) and neurotensin (NT) in gastric antrum and brain tissue of rat with chronic stress (CUMS) and to explore its mechanism.The experimental rat model of gastric motility disorders was replicated by chronic unpredictable mild stress (CUMS).The CUMS model rats were treated with different low dosages of Cap 0.04 mg/kg,0.4 mg/kg,4 mg/kg within two weeks.The gastric emptying rate of phenol red was used to reflect the gastric motility.The TRPV1 and NT content in gastric antrum and brain were measured by ELISA.The results showed that the gastric emptying rate of phenol red of CUMS model group was significantly lower than that of normal group.And after treating with different dosages of Cap,the gastric emptying rate of phenol red of each group were higher than that of CUMS model group (P<0.05).Moreover,with the increasing doses of Cap,the gastric emptying rate decreased.In addition,the increased expression of TRPV1 and NT were depended on the dosage of Cap.It can clearly be seen that the three doses of Cap showed an improve effect on the gastric motility disorders rats with CUMS,especially the 0.04 mg/kg dosage group.These results demonstrated that the low dosage of Cap might have an improve effect on the gastric motility disorders rats with CUMS,and one of the mechanisms was that Cap can regulate the expressions of TRPV1 and NT.

Key words: Capsaicin, gastric motility, TRPV1, NT

中图分类号: 

R573.5