天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (11): 1816-1821.doi: 10.16333/j.1001-6880.2016.11.024

• 开发研究 • 上一篇    下一篇

土茯苓叶和种子的抗氧化活性研究

朱晓娣1,张祥1,3,王红亚1,杨永秀1,田慧娟1,孙慧玲1,康文艺1,2*   

  1. 1 河南大学中药研究所;2开封市保健食品功效成分研究重点实验室;3河南应用技术职业学院,开封 475004
  • 出版日期:2016-11-30 发布日期:2017-03-30

Antioxidant Activity of Smilax glabra Roxb.Leave and Seed in vitro

ZHU Xiao-di1,ZHANG Xiang1,3,WANG Hong-ya1,YANG Yong-xiu1, TIAN Hui-juan1,SUN Hui-ling1,KANG Wen-yi1,2*   

  1. 1 Institute of Chinese Materia Medica,Henan University; 2 Kaifeng Key Laboratory of Functional Components in Health Food; 3Henan Vocational College of Applied Technology,Kaifeng 475004,China
  • Online:2016-11-30 Published:2017-03-30

摘要: 本文研究了土茯苓叶和种子的乙醇提取物不同组分的抗氧化活性。通过采用DPPH法、ABTS法、FRAP法、抗红细胞氧化溶血以及抑制肝肾组织中MDA的生成这5种体外抗氧化方法,并以BHA为阳性对照,发现土茯苓叶乙酸乙酯组分的清除DPPH自由基能力(IC50=21.15±2.90 μg/mL)、抗红细胞溶血(IC50=21.93±0.96 μg/mL),抑制肝肾组织中MDA生成(肝IC50=1.34±0.14 μg/mL,肾IC50=7.69±1.88 μg/mL)在土茯苓叶和种子不同组分中效果最好;土茯苓叶正丁醇组分的ABTS自由基的清除能力(IC50=2.29±0.19 μg/μL)和还原Fe3+的能力(Trolox当量=3768.44±16.93 μmol/g)效果最好,差异均具有统计学意义(P<0.05)。因此,土茯苓叶和种子均具有抗氧化活性,且叶的效果要优于种子,有望开发为一种抗氧化剂。

关键词: 土茯苓, 抗氧化, DPPH自由基, 抗溶血

Abstract: The antioxidant activity of different extracts of Smilax glabra leave and seed were investigated by DPPH,ABTS,FRAP,H2O2-induced red blood hemolysis assays and TBA method for determining the content of MDA in liver and kidney tissue in vitro. BHA was used as the positive control.The results showed that,in DPPH assays,the radical scavenging activity of S.glabra leave ethyl acetate extract (IC50=21.15±2.90 μg/mL) were good.The activity of anti-hemolysis and MDA inhibition in liver and kidney tissue of S.glabra leave ethyl acetate extract (IC50=21.93±0.96,1.34±0.14,7.69±1.88 μg/mL,respectively) were the higher than that of other extracts of S.glabra leave and seed.However,the best effect of ABTS radical scavenging activity and Trolox equivalent were the n-butanol extracts of S.glabra leave (ABTS IC50=2.29±0.19 μg/μL,and the value of Trolox equivalent was 3768.44±16.93 μmol/g).These results showed that both leave and seed of S.glabra had antioxidant activity.The antioxidant activity of S.glabra leave was higher than that of seed.It can be used as a potential antioxidant agent in food industry.

Key words: Smilax glabra Roxb., DPPH free radical, antioxidant, anti-hemolysis

中图分类号: 

R285