关键词: 茶蜂花粉, 抗氧化, 黄酮, 结构鉴定

Abstract: This experiment was aimed to study the antioxidant activity and functional components of camellia bee pollen.Camellia bee pollen was powdered and extracted by 85% ethanol under reflux and then was extracted by petroleum ether,ethyl acetate and n-butyl alcohol.D-Galactose induced aging mice model was used to detect the antioxidant activity of different components of camellia bee pollen.Various chromatographic methods were employed to separate the ingredients with antioxidant activity.Nuclear magnetic resonance (NMR) was applied to identify the chemical structure of the monomers.The results showed that the camellia been pollen ethanol extracts and ethyl acetate extracts had obvious antioxidant effect.Ethyl acetate extracts by MCI column chromatography was isolated into 30%,50%,70% ethanol elution contents.30% Ethanol elution had obvious antioxidant activity.Three monomer compounds were separated and identified from the elution part:quercetin,kaempferide-3-O-rutinoside and kaempferol-3-O-(2″,3″,4″-O-hydroxyl cinnamon acyl)-β-D-glucopyranoside.Therefore,flavonoids were the main functional components in camellia bee pollen for the antioxidant activity.

Key words: camellia bee pollen, antioxidant activity, flavonoids, structural identification