关键词: 黄芩, 黄芩黄酮总苷元, 黄芩素, 黄芩酶, pH值, 提取方法

Abstract: To develop a new method of obtaining Scutellaria Radix total flavone aglycone.The pH of baicalinasewas optimized using enzymatic hydrolysis rate of baicalin as evaluation index.Taking extraction yield of baicalin as evaluation index,parameters such as water volume,decocting time and extraction times were optimized by orthogonal test.The established method:stirring the Scutellariae Radix powder with water (pH3) for 0.5 h,then baicalinase solution was obtained by filtration,the residue was put into boiled water (12 times of residue) two times for decocting at pH6,0.5 h each time,then mixed the filtration fluid with the baicalinase solution at 50 ℃(pH6) for 6 h.Scutellaria Radix total flavone aglycon can be obtained by drying the precipitate of filtration.The extraction rate of baicaleinwas 80%.The developed method was simple,reliable and stable.It can be used as a way to get Scutellaria Radix total flavone aglycon from Scutellaria Radix.

Key words: Scutellaria baicalensisi, Scutellaria Radix totalflavone aglycone, baicalein, Scutellaria Radix enzyme, pH, extraction