天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (2): 262-265.doi: 10.16333/j.1001-6880.2016.2.017

• 开发研究 • 上一篇    下一篇

外源绿原酸对大豆下胚轴不定根形成的影响

刘江,陈中说,吴春,吴海军,张晨州,陈建华,邓俊才,秦雯婷,杨文钰*,陈兴福*   

  1. 四川农业大学农学院 农业部西南作物生理生态与耕作重点实验室,成都 611130
  • 出版日期:2016-02-29 发布日期:2016-03-07

Effect of Exogenous Chlorogenic Acid on Adventitious Rooting of Soybean Hypocotyl

LIU Jiang,CHEN Zhong-shuo,WU Chun,WU Hai-jun,ZHANG Chen-zhou,CHEN Jian-hua,DENG Jun-cai,QIN Wen-ting,YANG Wen-yu*, CHEN Xing-fu*   

  1. Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China
  • Online:2016-02-29 Published:2016-03-07

摘要: 以大豆下胚轴为试验材料,研究了绿原酸(CGA)及其与植物生长调节剂(IAA、IBA)共同作用对大豆下胚轴不定根发生的影响。结果表明,当CGA施用量达到200 μmol/L及400 μmol/L时,大豆下胚轴生根率分别显著降低了17.8%和26.7%,平均根长分别显著降低了18.7%和35.4%;当植物生长调节剂施用量为2 μmol/L时,可显著缓解CGA(100 μmol/L)对大豆不定根的抑制作用,使其生根率分别提高12.5%和20.0%,随着生长素施用量的进一步增加(10~50 μmol/L),其生根率显著下降;大豆不定根培养24 h内,对照下胚轴基部木质素相对含量降低,CGA处理大豆的木质素相对含量升高。高浓度CGA对大豆不定根形成具有抑制作用,其通过与木质素、生长素的共同作用,实现对大豆不定根发生的调控。

关键词: 绿原酸, 大豆下胚轴, 不定根, 抑制

Abstract: The effect of chlorogenic acid (CGA) and IAA/IBA on adventitious rooting of soybean hypocotyl was explored in this study.The results indicated that after treatment with 200 and 400 μmol/L CGA,rooting rate of soybean hypocotyl significantly decreased (17.8% and 26.7%,respectively),average root length significant decreased (18.7% and 35.4%,respectively);The inhibition effect of CGA on the adventitious rooting of soybean hypocotyl can be remitted by application of 2 μmol/L IAA/IBA,their rooting rate improved significantly (12.5% and 20.0%,respectively);with the increasing doses of IAA/IBA applications (10~50 μmol/L),the rooting rate significant decreased.Lignin content of soybean hypocotyl decreased in the first 24 h after treatment.The lignin content of samples suffered from 100 μmol/L CGA,increased inversely.High concentration of CGA treatment inhibited the growth of adventitious rooting of soybean.Low concentration of IAA/IBA treatment can remit the inhibition,high level of IAA/IBA treatment increased inhibition.In conclusion,CGA itself might not directly involve in organogenesis,but,might be related to a balance between IAA/IBA and lignin that control organogenesis of adventitious rooting in soybean hypocotyl.

Key words: chlorogenic acid, soybean, adventitious root, inhibition

中图分类号: 

Q946.8