关键词: 大苞短毛唇柱苣苔, 离体培养, 快速繁殖

Abstract: In this study,the condition of in vitro culture and rapid propagation of Chirita brachytricha.var. magnibracteata W.T.Wang et D.Y.Chen was investigated with its leaves as explants.The effects of different hormones for callus induction and plant regeneration of leaves were studied.The results showed that:the optimal way to obtain sterile explant for leaves were sterilized in 75% ethyl alcohol for15 s then 0.1% HgCl₂ for 4 min;The optimal medium for callus of leaves was MS+TDZ0.5 mg/L+NAA0.1 mg/L,induction rate was 83.75%;The optimal medium of induction of adventitious buds was MS+6-BA0.5 mg/L+NAA0.1 mg/L,Multiplication factor was 16,and seedlings grew well;The optimal medium and plant growth substances combination for rooting induction was 1/2MS+IBA0.1 mg/L;Rooting rate was 96.67%.Transplant survival rate of plantlet reached more than 95%.In conclusion,the condition of plant regeneration and clonal propagation system was established.It provided technical guidance for production of C.brachytricha.var.magnibracteata W.T.Wang et D.Y.Chen.

Key words: Chirita brachytricha.var. magnibracteata W.T.Wang et D.Y.Chen, in vitro  culture;rapid propagation