发酵罐发酵腐皮镰刀菌株产出HMG-CoA还原酶抑制剂的研究

doi:10.16333/j.1001-6880.2016.3.007

天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (3): 359-365.doi: 10.16333/j.1001-6880.2016.3.007

发酵罐发酵腐皮镰刀菌株产出HMG-CoA还原酶抑制剂的研究

孟哓琴1，吴文惠2,3，周喻1，付强1，郭锐华1，包斌1,2*

1 上海海洋大学食品学院；2 上海水产品加工及贮藏工程技术研究中心；3 上海海洋大学海洋科学研究院，上海 201306

出版日期:2016-03-30 发布日期:2016-04-07

Production of Fusarium solani strains by Fermentation Tank and Its Inhibition Effect on HMG-CoA Reductase

MENG Xiao-qin1,WU Wen-hui2,3,ZHOU Yu1,FU Qiang1,GUO Rui-hua1,BAO Bin1,2*

1 College of Food Science and Technology,Shanghai Ocean University;2 Shanghai Engineering Research Center of Aquatic Product Processing & Preservation;3 Institutes of Marine Science,Shanghai Ocean University,Shanghai 201306,China

Online:2016-03-30 Published:2016-04-07

摘要/Abstract

摘要： 以提高海洋微生物腐皮镰刀菌FG319产出HMG-CoA还原酶抑制剂MFS（Metabolite of Fusarium solani FG319）的数量为目标，优化摇瓶发酵培养基和培养条件，基于摇瓶条件设置发酵罐发酵参数获得MFS。使用GSB培养基、CYM培养基、ATCC培养基、BPY培养基、TYG培养基、OA培养基、CD培养基、PDB培养基，基于摇瓶发酵条件优化发酵罐发酵参数，用HPLC检测MFS的产出量，通过发色底物法评价MFS的HMG-CoA还原酶抑制活性。海洋微生物腐皮镰刀菌FG319的最佳培养基为CD培养基，发酵第7 d HMG-CoA还原酶抑制剂MFS的产量达到最大，诱导物L鸟氨酸盐酸盐提高了4.07倍MFS的产出量，全糖型、pH=6.9、不添加NaCl时海洋微生物腐皮镰刀菌FG319发酵罐发酵产出MFS的数量达到23.47 mg/L，体外评价体系显示MFS抑制HMG-CoA还原酶的活性约是洛伐他汀活性的1.38倍、是普伐他汀活性的1.74倍。通过培养基选择和发酵条件优化，显著提高了海洋微生物腐皮镰刀菌FG319发酵罐发酵产出HMG-CoA还原酶抑制剂的数量。

关键词: 培养条件, 腐皮镰刀菌FG319, HMG-CoA还原酶

Abstract: The goal of the present study was to improve the production of HMG-CoA reductase inhibitors MFS (Metabolite of Fusarium solani FG319),through optimizing the fermentation mediums and conditions of marine microorganism Fusarium solani FG319.GSB medium,CYM medium,ATCC medium,BPY medium,TYG medium,Oatmeal medium,Czapek-Dox (CD) medium and potato dextrose broth culture medium were used to optimize the fermentation conditions by shake flask fermentation and fermenter.The production of MFS was analyzed using a HPLC by chromogenic substrate.In addition,an inhibitory effect of MFS on HMG-CoA reductase was determined in vitro. The results showed that CD medium was the best medium for marine microorganism Fusarium solani FG319;maximum production of HMG-CoA reductase inhibitor MFS was achieved on 7^thday;inducer L-ornithine hydrochloride had increased the production of MFS (4.07 times);in all sugar type,the marine microorganism Fusarium solani FG319 metabolic MFS quantity was up to 23.47 mg/L without NaCl (pH = 6.9); in vitro evaluation system showed inhibition activity of MFS on HMG-CoA reductase was higher than the activity of lovastatin (1.38 times) and pravastatin (1.74 times).The medium selection and optimization of fermentation conditions significantly improved the production of HMG-CoA reductase inhibitor from marine microorganism Fusarium solani FG319.

Key words: optimization, culture conditions, Fusarium solani FG319, HMG-CoA reductase

中图分类号:

Q93-3

孟哓琴1，吴文惠2,3，周喻1，付强1，郭锐华1，包斌1,2*. 发酵罐发酵腐皮镰刀菌株产出HMG-CoA还原酶抑制剂的研究[J]. 天然产物研究与开发, 2016, 28(3): 359-365.

MENG Xiao-qin1,WU Wen-hui2,3,ZHOU Yu1,FU Qiang1,GUO Rui-hua1,BAO Bin1,2*. Production of Fusarium solani strains by Fermentation Tank and Its Inhibition Effect on HMG-CoA Reductase[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(3): 359-365.

发酵罐发酵腐皮镰刀菌株产出HMG-CoA还原酶抑制剂的研究

Production of Fusarium solani strains by Fermentation Tank and Its Inhibition Effect on HMG-CoA Reductase

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