天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (7): 1116-1120.doi: 10.16333/j.1001-6880.2016.7.022

• 开发研究 • 上一篇    下一篇

山茱萸多糖大孔树脂脱色技术的优化

雷呈1*,王桂桢2   

  1. 1 南阳医学高等专科学校;2 南阳农业职业学院,南阳 473000
  • 出版日期:2016-07-30 发布日期:2017-01-17

Optimization of Decolorization Technology of Cornus officinalis Sieb.et Zucc Polysaccharide by Macroporous Resin

LEI Cheng1*,WANG Gui-zhen2   

  1. 1 Nanyang Medical College;2Nanyang Vocational College of Agriculture,Nanyang 473000,China
  • Online:2016-07-30 Published:2017-01-17

摘要: 以脱色率、多糖保留率和细胞抑制率3个指标,从D301、D303、D315、D900树脂筛选出脱色效果较好的树脂;采用MTT法检测,脱色后的山茱萸粗多糖抗HL60细胞活性提高;对D303树脂,通过单因素和正交试验对其进行优化,确定最优工艺条件是时间7 h、温度50 ℃、样品浓度15 mg/mL,脱色率和多糖保留率分别为79.71%、81.12%。大孔树脂脱色工艺的研究,为山茱萸药物的研发提供了一定实验基础。

关键词: 山茱萸多糖, 大孔树脂, 脱色, 优化

Abstract: The resin with better decolorization effect was screened out from D301,D303,D315 and D900 resin using decolorization rate,polysaccharide retention rate and cell inhibition rate as evaluation indexes.The inhibition effects of Cornus officinalis Sieb.et Zucc polysaccharide before and after decolorization on HL-60 cells were investigated by MTT assay.D303 resin showed the best decolorization effect.Its processing conditions were further optimized by single factor and orthogonal experiments.The results showed that the optimal process condition were as follows:processing time of 7 h,temperature of 50 ℃,sample concentration of 15 mg/mL.Under the optimized conditions,the decolorization rate and polysaccharide retention rate were 79.71% and 81.12%,respectively.The developed decolorization technology using macroporous resin for C.officinalis polysaccharide provided experimental basis for its further research and development.

Key words: Cornus officinalis Sieb.et Zucc polysaccharide, macroporous resin, decolorization, optimization

中图分类号: 

R34