天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (8): 1289-1295.doi: 10.16333/j.1001-6880.2016.8.021

• 开发研究 • 上一篇    下一篇

AB-8树脂对蓝靛果花色苷的吸附性能研究

李良玉1,2,宋大巍3,刁静静1,贾鹏禹1,张丽萍1,3*   

  1. 1 黑龙江八一农垦大学国家杂粮工程技术研究中心,大庆 163319;2 东北林业大学林学院,哈尔滨 150040;3黑龙江八一农垦大学食品学院,大庆 163319
  • 出版日期:2016-08-30 发布日期:2017-01-17

Adsorption Characteristics of AB-8 Resin for Anthocyanins from Lonicera edulis Turcz

LI Liang-yu1,2,SONG Da-wei3,DIAO Jing-jing1,JIA Peng-yu1,ZHANG Li-ping1,3*   

  1. 1 Heilongjiang Bayi Agricultural University,National Coarse Cereals Engineering Research Center,Daqing 163319,China; 2 College of Forestry,Northeast Forestry University,Harbin 150040,China; 3 The Food Science of Heilongjiang Bayi Agricultural Universitiy,Daqing 163319,China
  • Online:2016-08-30 Published:2017-01-17

摘要: 以AB-8树脂分离蓝靛果花色苷的吸附性能为研究内容,具体研究了AB-8树脂的吸附等温线、吸附热力学性质和动态吸附参数。结果表明,在静态吸附实验条件下,AB-8树脂对蓝靛果花色苷的吸附是放热过程,吸附能力较强,吸附参数能用Freundlich方程较好拟合,相关系数大于0.98;动态吸附研究表明,AB-8树脂纯化蓝靛果花色苷的最佳上样浓度为280±5 mg/mL、洗脱流速2.5 mL/min,通过对乙醇梯度洗脱浓度进行液质分析,确定了各洗脱液中的花色苷种类,为模拟移动床色谱纯化蓝靛果花色苷奠定了理论基础。

关键词: AB-8树脂, 蓝靛果, 花色苷, 吸附热力学, 吸附等温线

Abstract: In this study,the adsorption properties of AB-8 resin for anthocyanins from Lonicera edulis Turcz were investigated.The adsorption isotherm,thermodynamic properties and dynamic parameters of AB-8 resin were analyzed.The results indicated that under experimental conditions of static adsorption,the adsorption of AB-8 resin for anthocyanins from L.edulis was an exothermic process,and the adsorption capacity was strong,the adsorption parameters fit Freundlich equation well with correlation coefficient greater than 0.98.The results of dynamic adsorption showed that the optimal feed concentration was 280±20 mg/mL,elution speed was 2.5 mL/min.The eluting liquid of anthocyanin varieties were analyzed and identified using LC-MS.The results of this study provided a theoretical foundation for the purification anthocyanins from L.edulis using simulated moving bed chromatography.

Key words: AB-8 resin, Lonicera edulis Turcz, anthocyanins, adsorption thermodynamics, adsorption isotherm

中图分类号: 

S713