关键词: 无梗五加, 抗肿瘤作用, 细胞因子

Abstract: To study the inhibitory effect of Acanthopanax sessiliflorus (Ruqr.et Maxim) Seem.fruit extract on tumor growth and the levels of cytokines,mice weight,thymus and spleen index in tumorbearing mice.H22 tumorbearing mice model was induced by H22 cell vaccination in normal mouse forelimb right axillary subcutaneous (0.2 mL/rat),then 90 KM mice were randomly divided into normal group (constant volume of distilled water),model group (constant volume of distilled water),cyclophosphamide group (0.03 g/kg),and the refined A.sessiliflorus polysaccharides highdose and lowdose groups (0.80,0.20 g/kg),the crude A.sessiliflorus polysaccharides highdose and lowdose groups (1.04,026 g/kg),the A.sessiliflorus ethanol extract highdose and lowdose groups (0.92,0.23 g/mL).They were given medicine intragastrically once a day for consecutive 10 days.The levels of IL2,IL6 and TNFα in tumorbearing mice were investigated by ELISA assay,the tumor weight and the tumor inhibition rate were calculated and the weight of thymus and spleen were weighted and the indexes of them were calculated.Compared with normal group,the contents of IL2,IL6,TNFα thymus and spleen index decrased significantly in model group.While tumor weight increased,there was statistical significance (P<0.05);Compared with model group,the A.sessiliflorus ethanol extracts highdose and lowdose groups,the crude A.sessiliflorus polysaccharides highdose groups,the refined A.sessiliflorus polysaccharides highdose and lowdose groups had marked inhibitory effects on tumor growth in vivo,the inhibition rates of A.sessiliflorus fruit extract on H22 was 53.27%,52.87%,51.98%,31.64%,38.14%,especially the ethanol extracts from A.sessiliflorus was the strongest one for anticancer activity.It could increase spleen index and thymus gland index and enhance the expression levels of IL2,IL6 and TNFα in tumorbearing mice,there was statistical significance (P<0.05).The ethanol extracts from A.sessiliflorus and polysaccharides can inhibit tumor growth of H22 tumorbearing mice.

Key words: Acanthopanax sessiliflorus, antitumor effect, cytokines