不同块菌的系统发育分析和对小鼠的免疫调节作用

doi:10.16333/j.1001-6880.2017.10.008

天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (10): 1677-1682.doi: 10.16333/j.1001-6880.2017.10.008

不同块菌的系统发育分析和对小鼠的免疫调节作用

金鑫1，陈诚2，陈祖琴1，黄文丽1*

¹四川省农业科学院生物技术核技术研究所，成都 610061；²四川省农业科学院植物保护研究所，成都 610066

出版日期:2017-10-27 发布日期:2017-10-27
基金资助:
四川省科技支撑计划（2016GZ0355）

Phylogenetic Analysis on Different Truffles and Immunomodulatory Effects on Mice

JIN Xin1, CHEN Cheng2, CHEN Zu-qin1, HUANG Wen-li1*

¹Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610061, China; ²Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaThe aim of this study was to analyzethe effect of Tuber aestivum,Tuber indicum and Tuber panzhihuanense on the immune function of mice.The three kinds of fresh truffles were made into homogenate.Different doses of truffle homogenate (125 and 2.5 g/kg) were administered once daily for two consecutive weeks,with normal saline as control group.The phagocytosis ability of murine macrophages was detected by neutral phagocytosis test.ConA-induced mouse spleen lymphocyte proliferation index was measured by MTT assay.Half-hemolytic value (HC50) was detected after SRBC immunization.The humoral immune function of mice was measured.The results showed that,T.indicum and T.panzhihuanense test group can significantly improve the phagocytosis of macrophage of mice,the low dose of T.panzhihuanensegroup was most significant,and three kinds of truffles significantly enhanced the proliferation of mouse spleen lymphocytes.The best effect of high dose groupwas T.indicum.At the same time the content of serum hemolysin were all substantially improved in mice.The difference between each experimental group was not significant (P<0.05).The three kinds of fresh truffle homogenates can significantly enhance the immunity of the body,and the comprehensive analysis showed that the high dose group of T.indicumiswas more effective.

Online:2017-10-27 Published:2017-10-27

摘要/Abstract

摘要： 研究分析夏块菌（Tuber aestivum）、印度块菌（Tuber indicum）和白块菌（Tuber panzhihuanense）对小鼠免疫功能的调节作用。将三种新鲜块菌制成匀浆液，按照高剂量（125 g/kg）、低剂量（2.5 g/kg）的块菌经灌胃不同剂量的块菌匀浆，每天灌胃一次，连续2周，以生理盐水为对照组。分别通过中性红的吞噬实验来检测小鼠巨噬细胞的吞噬能力，MTT法测定ConA诱导的小鼠脾淋巴细胞增值指数，SRBC免疫小鼠后检测其半数溶血值（HC₅₀），测定小鼠的体液免疫功能。结果显示夏块菌、印度块菌和白块菌实验组都能显著提高小鼠巨噬细胞的吞噬能力，其中低剂量组的白块菌效果最显著，三种块菌匀浆都显著增强了小鼠脾淋巴细胞的增殖作用，高剂量组的印度块菌效果最好，同时都大幅提高小鼠血清溶血素的含量，各实验组之间的差异不显著（P<0.05）。研究表明这三种新鲜块菌匀浆能够显著增强机体的免疫力，综合分析得出印度块菌的高剂量组效果更显著。

关键词: 夏块菌, 印度块菌, 白块菌, 系统发育分析, 免疫功能

Abstract: The aim of this study was to analyzethe effect of Tuber aestivum , Tuber indicum and Tuber panzhihuanense on the immune function of mice.The three kinds of fresh truffles were made into homogenate.Different doses of truffle homogenate (125 and 2.5 g/kg) were administered once daily for two consecutive weeks,with normal saline as control group.The phagocytosis ability of murine macrophages was detected by neutral phagocytosis test.ConA-induced mouse spleen lymphocyte proliferation index was measured by MTT assay.Half-hemolytic value (HC₅₀) was detected after SRBC immunization.The humoral immune function of mice was measured.The results showed that, T.indicum and T.panzhihuanense test group can significantly improve the phagocytosis of macrophage of mice,the low dose of T.panzhihuanense group was most significant,and three kinds of truffles significantly enhanced the proliferation of mouse spleen lymphocytes.The best effect of high dose groupwas T.indicum. At the same time the content of serum hemolysin were all substantially improved in mice.The difference between each experimental group was not significant (P<0.05).The three kinds of fresh truffle homogenates can significantly enhance the immunity of the body,and the comprehensive analysis showed that the high dose group of T.indicumiswas more effective.

Key words: Tuberaestivum, Tuber indicum, Tuber panzhihuanense; phylogenetic analysis, immune function

中图分类号:

S859.7

金鑫1，陈诚2，陈祖琴1，黄文丽1*. 不同块菌的系统发育分析和对小鼠的免疫调节作用[J]. 天然产物研究与开发, 2017, 29(10): 1677-1682.

JIN Xin1, CHEN Cheng2, CHEN Zu-qin1, HUANG Wen-li1*. Phylogenetic Analysis on Different Truffles and Immunomodulatory Effects on Mice[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2017, 29(10): 1677-1682.

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不同块菌的系统发育分析和对小鼠的免疫调节作用

Phylogenetic Analysis on Different Truffles and Immunomodulatory Effects on Mice

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