关键词: 蜂毒肽, 树突细胞, T细胞, 免疫调节

Abstract: To study the intervention of melittin (MT) on dendritic cells (DC) co-cultured with lymphocytes stimulated by lipopolysaccharide (LPS),the impact of MT on the differentiation of Th1/Th2 was investigated.MTS was used to detect the activity of DC co-cultured with lymphocytes by LPS and MT;Flow cytometry was used to detect the ratio of Th1 and Th2 in the co-culture of DCs stimulated by MT and LPS;ELISA was used to detect the concentration IL-4,INF-γ in cell supernatant of MT and LPS stimulated co-cultured DC and lymphocyte.The results showed that a certain concentration of MT and LPS can stimulate the co-cultured activity of DC with lymphocytes.In the induction of mature DC,MT can increase the Th1/Th2 ratio,the Th1 percentage (P<0.05) and the IFN-γ (P<0.01).But it had no effect on the concentration of IL-4 and Th2 (P>0.05).The study demonstrated that through upregulating the expression of Th1 cell ratio and IFN-γ concentration,MT promoted the differentiation of Th1/Th2 cells to the Th1 pathway,thus playing immunomodulatory effects on T cells.

Key words: melittin, dendritic cell, T cell, immune regulation