天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (3): 425-430.doi: 10.16333/j.1001-6880.2017.3.011

• 研究论文 • 上一篇    下一篇

麻欠产地对精油化学成分及抗炎效果的影响

董丽华1,2，郭娟1，张红霞3，杜芝芝3，许又凯1，张萍1*   

  1. 1 中国科学院西双版纳热带植物园热带植物资源可持续利用重点实验室，西双版纳 666303；2中国科学院大学，北京 100049；3中国科学院昆明植物研究所，昆明 650201
  • 出版日期:2017-03-31 发布日期:2017-04-06

Chemical Composition and Anti-Inflammatory Effects of Essential Oil of Maqian(Zanthoxylum myriacanthum  var. pubescens Huang) Collected in Three Locations of Xishuangbanna,Yunnan

DONG Li-hua1,2,GUO Juan1,ZHANG Hong-xia3,DU Zhi-zhi3,XU You-kai1,ZHANG Ping1*   

  1. 1 Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical  Botanical Garden,Chinese Academy of Sciences,Xishuangbanna,666303,China;2Univercity of  Chinese Academy of Science,Beijing 100049,China;3Kunming Institute of  Botany,Chinese Academy of Sciences,Kunming 650201,China
  • Online:2017-03-31 Published:2017-04-06

PDF (PC)

296

摘要： 为了进一步探究麻欠的产地对精油成分和抗炎效果的影响，本研究针对从西双版纳州的勐仑、勐旺、勐醒三个地点采集的麻欠果实，以水蒸馏法提取精油，用气相色谱-质谱（GC-MS）联用技术分析化学成分并用RAW 264.7细胞研究抗炎效果。精油共鉴定出21种化合物，以烯、醇类物质组成为主。其中勐仑与勐醒所产精油成分含量相似，与勐旺所产精油差异较大。溶剂对照、地塞米松或麻欠精油预处理RAW264.7细胞后，LPS处理培养24 h后，测定NO、TNF-α的含量。三地精油对LPS刺激产生的NO和TNF-α均有显著抑制作用。结果表明，虽然三个地点的麻欠精油成分有差异，但是抗炎效果相当。

关键词: 麻欠精油, 产地差异, 化学成分, RAW264.7, 抗炎

Abstract: In order to understand the effects of location on the chemical composition and anti-inflammatory effects of Maqian(Zanthoxylummyriacanthum var.pubescens Huang) essential oil(MQEO),fruits of Maqian were collected from three locations in Xishuangbanna(Menglun,Mengwang,Mengxing).The essential oils were obtained by hydrodistillation.The chemical composition was analyzed using GC-MS and the anti-inflammatory effect was determined by RAW264.7 cells.A total of 21 kinds of substances were identified in three MQEOs among which alkenes and alcohols were the dominant components.The chemical composition of MQEOs from Menglun and Mengxing was more similar,whereas MQEO from Mengwang showed marked difference in the content of several major components from those in Menglun and Mengwang samples.RAW264.7 cells were pretreated with vehicle,Dex,or MQEO,and then cultured with LPS for 24 h.NO was determined by Griess reagent.TNF-α was determined by ELISA.Three MQEO all exhibited dose-dependent inhibition of LPS-stimulated production of NO and TNF-α.These results showed that despite difference in the chemical composition of MQEO from three locations,their anti-inflammatory effects remain the same.

Key words: Maqian essential oil, producing area, chemical composition, RAW264.7, anti-inflammatory

中图分类号: 

R967

版权所有 © 《天然产物研究与开发》编辑部
地址：四川天府新区群贤南街289号　邮编：610299
电话：028-85210304　QQ群：342035423　Email：trcw@clas.ac.cn　
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量：　今日访问：　在线人数：