关键词: 澳洲茄碱, 胆管癌, 细胞凋亡

Abstract: This study focused on investigating the effect of solasonine on human cholangiocarcinoma epithelial QBC939 cells via inducing apoptosis and related mechanisms.MTT assay was used to detect the inhibition effect of solasonine on QBC939 cell proliferation,flow cytometry was applied to detect QBC939 cells apoptosis induced by solasonine,Western blot was used to detect the expression changes of apoptosis-related proteins(Bax,Bcl2,caspase3,caspase7,XIAP,PARP,cleaved PARP) induced by solasonine in QBC939 cells.The results showed that solasonine can inhibit the proliferation of QBC939 cells in a dose-dependent manner significantly,solasonine can induce apoptosis of QBC939 cells obviously,and solasonine can increase protein expression level of Bax,caspase3,caspase7 and cleaved PARP and decrease the protein expression level of Bcl-2,XIAP and PARP.Those results suggested that solasonine can induce apoptosis of cholangiocarcinoma QBC939 cells significantly by changing the expression of apoptosis-related protein,which had the potential to be the drug candidate for the treatment of human cholangiocarcinoma in future.

Key words: solasonine, cholangiocarcinoma, apoptosis