关键词: 地榆总皂苷, ⁶⁰Co-&gamma, 射线, 骨髓抑制, IL-3, GM-CSF, SCF, c-Kit

Abstract: Protective effects and mechanism of total saponins of Sanguisorba officinalis L.(TSSO) on myelosuppressivemiceinduced by ⁶⁰Co-γ ray was investigated.The myelosuppressivemice modelwas established by irradiating the whole body one time with 650 cGy ⁶⁰Co-γ  ray.Then the myelosuppressive mice were treated with TSSO(1,0.5 and 0.1 mg/kg,i.g.) once a day,for total 10 days.The levels of WBC,RBC,HCT,PLT and PCT were measured at the 4^th, 7^th and 10^th day.The next day,the liver,spleen and thymus was collected for counting organ index.The numbers of bone marrow cells were also counted and the mRNA expressions of GM-CSF,IL-3,SCF and c-Kit were measured by qPCR analysis.The results showed that TSSO can improve the levels of WBC,RBC,HCT,PLT,PCT,BMC,and the mRNA expression of GM-CSF,IL-3,SCF,and c-Kit in TSSO groups significantly.Thus,TSSO can alleviate myelosuppression induced by ⁶⁰Co-γ ray. The improvement of the mRNA expressions of GM-CSF,IL-3,SCF and c-Kit may be the mechanisms.

Key words: total saponins of Sanguisorba officinalis L.;⁶⁰Co-&gamma, ray;myelosuppression;IL-3;GM-CSF;SCF;c-Kit