关键词: &pi, 键二苯甲酮异构体, 环大叶藤黄醇和异大叶藤黄醇, 人面果, 高效液相色谱法, 制备分离

Abstract: This study reported a liquid chromatographic method for preparing π- bond benzophenone isomers cycloxanthochymol and isoxanthochymol from Garcinia xanthochymus.The liquid chromatographic method was proposed,simulated and optimized through column selection and buffer selection on analytical columns,and verified on a preparative column.Cycloxanthochymol and isoxanthochymol were prepared after the inject volume selection,and identified by NMR as well as mass spectrometry.The optimized method realized baseline separation with resolution of 2.5,and the isomers were prepared with the inject volume of 500 μL at sample concentration of 0.2 μg/μL.Results of the HPLC analysis and the distortionless enhancement by polarization transfer confirmed the differences between the monomers on the π- bond position.In conclusion,this study indicated that a common octadecyl bonded silica gel column can meet the challenge preparation of π-bond isomers,which will accelerate the foundation of more bioactive benzophenone compounds in the future.

Key words: &pi, -bond benzophenone isomer, cycloxanthochymol and isoxanthochymol, Garcinia xanthochymus, high-performance liquid chromatography, preparative separation