天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (8): 1355-1361.doi: 10.16333/j.1001-6880.2017.8.017

• 开发研究 • 上一篇    下一篇

基于色度分析原理的广陈皮贮藏年限判别

王智磊,伍清芳,刘素娟,张鑫,陈林,陈鸿平,刘友平*   

  1. 成都中医药大学药学院 中药材质量标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都 611137
  • 出版日期:2017-08-28 发布日期:2017-08-28

Appraising Storage Periods of Citrus reticulata ‘Chachi’ Based on Colorimetry

WANG Zhi-lei,WU Qing-fang,LIU Su-juan,ZHANG Xin,CHEN Lin,CHEN Hong-ping,LIU You-ping*   

  1. Pharmacy College,Chengdu University of Traditional Chinese Medicine,Breeding Base of State Key Laboratory of Resources System Research and Development Utilization of Chinese Herbal Medicines Co-constuctioned by Ministry of Science and Technology and Sichuan Province,Chengdu,611137,China
  • Online:2017-08-28 Published:2017-08-28

摘要: 探讨广陈皮颜色特征与贮藏年限之间的相关性及不同贮藏年限广陈皮质量评价。通过分光测色仪测定不同贮藏年限广陈皮颜色值L*、a*、b*,紫外-可见分光光度法测定总黄酮含量,高效液相色谱法(High Performance Liquid Chromatography,HPLC)测定芸香柚皮苷、橙皮苷、川陈皮素、桔红素含量。运用SPSS20.0软件对颜色值与贮藏年限、有效成分含量进行相关性分析。贮藏年限与颜色值L*、b*存在显著相关性,相关系数分别为-0.935、-0.917,即随着贮藏年限的增加,粉末颜色明度变暗并向蓝色方向移动;贮藏年限与颜色值L*、b*通过模型转化后存在直线回归关系,回归方程分别为Ln(颜色值L*)=4.240-0.089·Ln(贮藏年限)和Ln(颜色值b*)=3.220-0.156·Ln(贮藏年限);随着贮藏年限增加,总黄酮及4种黄酮类成分呈曲线波动型变化,其变化趋势基本一致。广陈皮贮藏年限与颜色值L*、b*存在显著相关性,为快速判别陈皮贮藏年限提供参考;受到药材质量难以统一的影响,以黄酮类成分变化探讨陈皮“陈久者良”欠佳。

关键词: 广陈皮, 颜色值, 贮藏年限判别, 陈久者良, 质量评价

Abstract: The aim of this study was to investigate the correlation between the color characteristics and the storage years of Citrus reticulata ‘Chachi’ (GCRP).The color values L*,a*,b* were determined by colorimeter.The content of total flavonoids was determined by UV spectrophotometry.The contents of narirutin,hesperidin,nobiletin and tangeretin were determined by HPLC.SPSS software was used to analyze the correlation between the color value,the storage years and the content of effective components.Results show that the storage years are significantly correlated with the color values of L* and b*.The correlation coefficients are -0.935 and -0.917,respectively.With the increase of storage years,the brightness of the powder color darkened and move into the blue direction.The regression equation are Ln (color value L*)=4.240-0.089 · Ln (storage years) and Ln (color value b*)=3.220-0.156 · Ln (storage years).With the increase of storage years,the total flavonoids and the four kinds of flavonoids show curve-type change and the change trend is basically the same.According to the results of the research,the storage years of GCRP have a significant correlation with the color value L*,b*.The color value of the medicinal materials can provide a reference for the rapid identification of the storage years.It is difficult to discuss “the older,the better” of GCRP from the changes of flavonoids because of the quality of the medicinal materials.

Key words: Citrus reticulata &lsquo, Chachi&rsquo, color value, discrimination of storage years, &ldquo, the older,the better&rdquo, quality evaluation