天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (1): 120-126.doi: 10.16333/j.1001-6880.2018.1.021

• 开发研究 • 上一篇    下一篇

兔毛角蛋白的制备及其在防晒化妆品中的应用

张毅,张睿,张昊*,黎淑婷   

  1. 天津工业大学纺织学部 先进纺织复合材料教育部重点实验室,天津 300387
  • 出版日期:2018-01-26 发布日期:2018-01-30
  • 基金资助:

    农业部财政部国家级兔产业技术体系建设专项(CARS-44-D-2);国家自然科学基金(5150030497)

Preparation of Rabbit Hair Keratin and Its Application in Sunscreen Cosmetics

ZHANG Yi,ZHANG Rui,ZHANG Hao*,LI Shu-ting   

  1. College of Textile;Key Laboratory of Advanced Textile Composites of Ministry of Education,Tianjin Polytechnic University,Tianjin 300387,China
  • Online:2018-01-26 Published:2018-01-30

摘要: 以丰富廉价的兔毛为原料,采用物理法提取兔毛角蛋白,将提取物作为应用于紫外防护保健品中的一种天然防晒素材。采用粒径分析,拉曼光谱、X射线衍射、氮元素及蛋白质含量测定、氨基酸含量分析、SDS-PAGE凝胶电泳对提取角蛋白进行了定性与定量分析研究。分析结果表明,提取角蛋白含量为89%~91%;粒径中值为100.99 nm,颗粒小,分布均匀;分子量较小,为10~20 KDa;物理法提取的角蛋白二级结构发生转变,有一定的β-折叠转变为ɑ-螺旋结构。紫外吸收测试结果表明以兔毛角蛋白为主要原料自制的天然防晒霜吸收紫外线性能较强且吸光度随配方中兔毛角蛋白含量的增加而提高,将其作为一种紫外防护功能添加剂应用于化妆品领域前景良好。兔毛角蛋白中含有丰富的芳香族氨基酸可能是其具有良好紫外吸收性能的主要原因。

关键词: 兔毛, 角蛋白, 提取, 紫外吸收, 防晒化妆品

Abstract: Abundant and cheap rabbit hair was used as raw material to extract rabbit hair keratin using physical method.The extracted rabbit hair keratin was used as a natural sunscreen material and was applied to UV protective health care products.The qualitative and quantitative analysis of extracted keratin was carried out by particle size analysis,Raman spectra (RAM),X-ray diffraction (XRD),nitrogen and protein content,amino acid content analysis and SDS-Polyacrylamide gel electrophoresis (SDS-PAGE).The results showed that the content of extracted keratin was 89%-91%,the median diameter was 100.99 nm,the particles were small and the distribution was uniform,the molecular weight was 10-20 KDa;the secondary structure of keratin extracted by physical method was changed,there was a certain β-sheet into ɑ-helix structure.UV absorption test results showed that the natural sunscreen with rabbit hair keratin as the main raw material to absorb ultraviolet light strong and absorbance with the formula in the rabbit hair keratin content increased,as a UV protective additive used in cosmetics field have a good prospect.Rabbit hair keratin is rich in aromatic amino acids may be a good reason for its excellent UV absorption.

Key words: rabbit hair, keratin, extraction, UV absorption, sunscreen cosmetics

中图分类号: 

TQ936.21+5