两株长喙壳属真菌挥发性香气成分分析

doi:A10.16333/j.1001-6880.2018.11.015

天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (11): 1939-1942.doi: A10.16333/j.1001-6880.2018.11.015

两株长喙壳属真菌挥发性香气成分分析

张志超1，张彧1，李建梅1，徐可成1，宋红1，李雪1，杨虹2*，黄琼1*

1云南农业大学省部共建云南生物资源保护与利用国家重点实验室，昆明 650201；2云南省农药检定所，昆明 650034

出版日期:2018-12-03 发布日期:2018-12-03
基金资助:
国家自然科学基金（31860522）；福建农林开放项目（NYST-2017-03）

The Aroma Components Analysis of Two Ceratocystis Strains

ZHANG Zhi-chao1, ZHANG Yu1, LI Jian-mei1, XU Ke-cheng1, SONG Hong1, LI Xue1, YANG Hong2*, HUANG Qiong1*

1State Key Laboratory of Conservation and Utilization of Biology Resources of Yunnan Province,Yunnan Agriculture University,Kunming,Yunnan 650201,China; 2Pesticide Inspection and Identification Institute of Yunnan,Kunming 650034,China

Online:2018-12-03 Published:2018-12-03

摘要/Abstract

摘要： 长喙壳属真菌具有产香特性，本文比较了长喙壳菌株PP1-1PG、WYB的菌落形态、rDNA-ITS序列等特征，并利用气相色谱-质谱（GC-MS）联用技术分析其有机化合物组成。结果表明，菌株在PSA培养基上菌丝不发达，为有隔菌丝。菌株PP1-1PG在ITS基因序列系统进化树上与Ceratocystis fimbriata聚为一支，菌株WYB与Ceratocystis paradoxa聚为一组。GC-MS分析表明，两个菌株产生的挥发性香气物质组成主要为乙酸乙酯、乙酸异丁酯、苯乙烯等，其中乙酸乙酯、乙酸异丁酯相对含量最高，二者之和在两个菌株中分别达到45.85%与61.02%。本研究首次在菌株PP1-1PG中检测到重要的香料物质苯甲醛（安息香醛），为开发利用苯甲醛提供了参考依据。

关键词: 长喙壳属真菌, ITS序列, 挥发性物质, GC-MS分析

Abstract: The genus Ceratocystis produces a range of flavoring compounds,in this study,strains PP1-1PG and WYB were compared on the basis of characteristics of colony morphology and ITS sequences.The volatile components were analyzed by gas chromatography-mass spectrometry (GC-MS).Septate mycelia were observed on PSA substrates for both strains.Based on the ITS phylogeny,strain PP1-1PG was clustered with Ceratocystis fimbriata-forming a distinct clade while strain WYB was clustered with Ceratocystis paradoxa.Analyzing the volatile components by GC-MS,it is known that the most materials produced by two strains were ethyl acetate,isobutyl acetate and styrene,which the relative contents of ethyl acetate and isobutyl acetate were 45.85% and 61.02% in two strains.Benzaldehyde,a widely used flavour substance,was detected from strain PP1-1PG for the first time,providing chemical basis and foundation for the comprehensive development and the search for this substance.

Key words: Ceratocystis spp., ITS sequence, volatile compounds, GC-MS analysis

中图分类号:

R284.1 Q939.95

张志超1，张彧1，李建梅1，徐可成1，宋红1，李雪1，杨虹2*，黄琼1*. 两株长喙壳属真菌挥发性香气成分分析[J]. 天然产物研究与开发, 2018, 30(11): 1939-1942.

ZHANG Zhi-chao1, ZHANG Yu1, LI Jian-mei1, XU Ke-cheng1, SONG Hong1, LI Xue1, YANG Hong2*, HUANG Qiong1*. The Aroma Components Analysis of Two Ceratocystis Strains[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2018, 30(11): 1939-1942.

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两株长喙壳属真菌挥发性香气成分分析

The Aroma Components Analysis of Two Ceratocystis Strains

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