天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (12): 2063-2069.doi: 10.16333/j.1001-6880.2018.12.005

• 研究论文 • 上一篇    下一篇

白兰花挥发油化学成分及其抑菌拒食活性研究

侯冠雄1,2，王永江2，张周鑫2，颜一军2，马亚团2，朱艳玲3*，黄胜雄1,2*   

  1. 1云南中医学院中药学院，昆明 650500；2中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室，昆明 650201；3昆明医科大学海源学院，昆明 650106；  
  • 出版日期:2019-01-02 发布日期:2019-01-02
  • 基金资助:

    云南省应用基础研究面上项目（2016FB021）；国家自然科学基金（81522044）

Study on the Volatile Oil of Michelia alba DC.and Its Antimicrobial and Antifeedant Activities

HOU Guan-xiong1,2,WANG Yong-jiang2,ZHANG Zhou-xin2,YAN Yi-jun2,MA Ya-tuan2,ZHU Yan-ling3*,HUANG Sheng-xiong1,2*     

  1. 1College of Pharmaceutical Science,Yunnan University of Traditional Chinese Medicine,Kunming 650500,China; 2State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China; 3Haiyuan College of Kunming Medical University,Kunming 650106,China
  • Online:2019-01-02 Published:2019-01-02

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摘要： 利用水蒸气蒸馏法提取白兰花中的挥发性成分，通过气相色谱质谱联用技术（GC-MS）并结合计算机分析鉴定提取物中挥发油芳香成分，共鉴定出76个化合物，其中有37个萜类化合物、17个醇类化合物、6个烷烃类化合物和其它类型化合物。用面积归一化法测定挥发油中各种成分的相对含量，合计占总峰面积的93292%。同时对不同菌株进行抑菌实验，并探究不同浓度挥发油对粘虫的拒食作用。实验结果表明：用水蒸气蒸馏提取白兰花挥发油时，提取率为4.18%，其最主要成分为芳樟醇；该方法提取的白兰花挥发油对水稻黄单胞菌和枯草芽孢杆菌抑制作用最好，并且对粘虫幼虫有很好拒食活性。

关键词: 白兰花, 挥发油, 拒食性, GC-MS, 抑菌活性, 粘虫

Abstract: Volatile oils were obtained from the flowers of Michelia alba DC.by means of steam distillation technique.The volatile components in the extracts were analyzed by GC-MS and their relative contents were identified by peak area normalization method.The results showed that there are 76 identified compounds which include 37 terpenoids,17 alcohols,6 alkane compounds and other substances in the essential oils.The relative contents of all detected compounds in the essential oils are 93.026%.In addition,we conducted antimicrobial experiments with different strains,and explored the antifeedant properties of essential oils in different concentrations against the worm Mythimna separata.The experimental results showed that the extraction rate was 4.18% by using steam distillation extraction method.The most abundant component of the extracted oil is linalool.The essential oils showed most potent inhibitory activity against Xanthomonas oryzae and Bacillus subtilis among the tested strains and it had a good inhibitory effect on the armyworm Mythimna separata.

Key words: Michelia alba DC., volatile oil, antifeedant, GC-MS, antimicrobial activity, Mythimna separata

中图分类号: 

R284.2 Q961

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