天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (12): 2146-2149.doi: 10.16333/j.1001-6880.2018.12.018

• 研究简报 • 上一篇    下一篇

益母草抗结核分枝杆菌成分的跟踪分离

韩立芬,宋善敏,甘梦兰,王仁凤,杨再昌*   

  1. 贵州大学药学院,贵阳 550025
  • 出版日期:2019-01-02 发布日期:2019-01-02
  • 基金资助:

    国家自然科学基金(81460531,81760629,81160390);黔科合人才团队[2015]4026号

Isolation and Identification of Anti-Mycobacterium tuberculosis Components from Leonurus artemisia (Laur.) S.Y.Hu F

HAN Li-fen, SONG Shan-min, GAN Meng-lan, WANG Ren-feng, YANG Zai-chang*   

  1. Pharmacy College of Guizhou University,Guiyang 550025,China
  • Online:2019-01-02 Published:2019-01-02

摘要: 结核病分为肺结核和肺外结核,其中子宫结核属于肺外结核,是造成不孕的主要原因。益母草是我国传统中药,常用于妇科疾病的治疗。在筛选中发现,益母草乙醇提取物具有抗结核分枝杆菌活性,经活性跟踪分离,从益母草乙酸乙酯萃取部位中分离得到具有抑制结核分枝杆菌的单体化合物1,鉴定为益母草碱,抗结核杆菌的MIC值为80 μg/mL。这一发现为治疗子宫结核提供了新的线索。

关键词: 益母草, 益母草碱, 结核分枝杆菌, 活性成分

Abstract: Tuberculosis is classified as pulmonary tuberculosis and extrapulmonary tuberculosis.The endometrial tuberculosis belongs to extrapulmonary tuberculosis,which is the main cause of infertility.Leonurus artemisia (Laur.) S.Y.Hu F is a traditional Chinese medicine,commonly used in the treatment of gynecologic diseases.In screening,it was found that the ethanol extract of Leonurus artemisia (Laur.) S.Y.Hu F had anti-Mycobacterium tuberculosis activity.Based on bioassay-guided isolation,compound 1 with the activity of inhibiting Mycobacterium tuberculosis was separated from the ethyl acetate extracts of Leonurus artemisia (Laur.) S.Y.Hu F.It was identified as leonurine,and with MIC value of 80μg/mL for against tubercle bacillus.This discovery might provide a new clue for the treatment of uterine tuberculosis.

Key words: Leonurus artemisia (Laur.) S.Y.Hu F, leonurine, mycobacterium tuberculosis, active component

中图分类号: 

R284.1 R93