关键词: 金银花尺蠖, 粪便提取物, 自由基, 抗氧化活性

Abstract: The excessive accumulation of free radicals would affect the normal function of the organism,hence it is very important for developing sandy tea to select insect metabolites with free radical scavenging function.In order to know the antioxidant activity of extracts from larva feces of Heterolocha jinyinhuaphaga Chu,the antioxidant activity of alcohol extracts from larva feces of H.jinyinhuaphaga in vitro was tested in the laboratory.The results showed that,extracts from larva feces of H.jinyinhuaphaga had a certain clearing effects on DPPH,·OH,O-·² and ABTS⁺·.At the concentration of 0.05 mg/mL,its clearing rate on them were 37.78%,25.31%,21.41% and 35.82% separately.With the increasing of concentrations,the clearing rate on them increased,and reached 95.52%,69.07%,63.27% and 72.46% at 0.65 mg/mL,which increased 57.74%,43.76%,41.86% and 36.64% separately,the difference between them reached significant level.The extracts also had a certain prevention effects on peroxidation of yolk lipoprotein with a preventing rate of 26.78% at the concentration of 0.05 mg/mL,it would also increase with the concentrations added,and reached 5063% at 0.65 mg/mL,which added 23.85%,the difference between them reached significant level.From 0.05 mg/mL to 0.65 mg/mL,the clearing rate on free radicals and prevention rate on peroxidation of yolk lipoprotein all showed a linear connection with the concentrations.Altogether,extracts from larva feces of H.jinyinhuaphaga had the potentiality to be exploited as sandy tea.

Key words: Heterolocha jinyinhuaphaga, feces extract, free radicals, antioxidant activity