关键词: 黑果腺肋花楸, 超声波, 花色苷, 响应面法, 稳定性

Abstract: The extraction conditions for improved yield of anthocyanins from Black Chokeberry (Aronia melanocarpa) were optimized by single factor experiment and response surface method.The stability of anthocyanins was investigated at the same time.The optimal extraction conditions of anthocyanins from Black Chokeberry were as follows:ethanol concentration was 63%,extraction time was 32 min,extraction temperature was 42 ℃,solid-liquid ratio was 1∶20 (g/mL).And the extraction yield of anthocyanins under these conditions was 5.61 mg/g.As the temperature was lower than 50 ℃,the preservation rate of anthocyanins in the crude extract was above 98%.However,the oxidant had a significant effect on the stability of anthocyanins while the stability of anthocyanins was best in dark environment.Ca²⁺,K⁺ and Mg²⁺ had little destructive effect on the anthocyanins while Fe²⁺ and Cu²⁺ had significant destructive effect on the anthocyanins.In addition,anthocyanins were more stable under acidic conditions.This study provided important information for the extraction of anthocyanins from Black Chokeberry.

Key words: Black Chokeberry (Aronia melanocarpa), ultrasonic, response surface, anthocyanins, stability