天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (4): 541-546.doi: 10.16333/j.1001-6880.2018.4.002

• 研究论文 • 上一篇    下一篇

当归产区根际土壤近红外漫反射光谱指纹图谱研究

顾志荣1,2,3,沈丹丹1,张瑛1,3,萨日娜2,3,刘洁丽1,3,王亚丽1,3*   

  1. 1甘肃中医药大学;2甘肃省人民医院药剂科;3甘肃省中药质量与标准研究重点实验室培育基地,兰州 730000
  • 出版日期:2018-05-07 发布日期:2018-05-07
  • 基金资助:

    国家自然科学基金(30960037);甘肃省人民医院青年科研项目(16GSSY7-2);甘肃省发改委战略新兴产业和产业技术研究与开发专项(2011);甘肃省科技支撑计划(144FKCA078)

     

Study on Near Infrared Diffuse Reflectance Spectrum Fingerprints of Rhizosphere Soil from Angelica Sinensis

GU Zhi-rong1,2,3, SHEN Dan-dan1, ZHANG Ying1,3, SA Ri-na2,3, LIU Jie-li1,3, WANG Ya-li1,3*   

  1. 1Gansu University of TCM; 2Department of Pharmacy, Gansu Provincial Peoples Hospital; 3Key Laboratory of Quality and Standard of TCM in Gansu Province, Lanzhou 730000,China
  • Online:2018-05-07 Published:2018-05-07

摘要: 以积分球漫反射方式采集11个当归产区共147批根际土壤样品的NIRDRS,解析特征吸收峰,采用“相关系数法”对NIRDRS指纹图谱进行相似度分析,建立正交偏最小二乘-判别分析(OPLS-DA)模型探讨其与土壤类型及产区划分的关系。结果表明8个主产县根际土壤的NIRDRS指纹图谱、一阶导数光谱及二阶导数光谱均较为相似,但临洮县、临潭县及康乐县等3个产区与其他产地(尤其是岷县)差异明显;基于OPLS-DA模型能够将各产区根际土壤的NIRDRS指纹图谱进行明确区分,其差异的主要波数范围为7352.5 ~ 6935.9 cm-1、5819.9 ~ 5760.4 cm--1、4927.1 ~4609.7 cm-1、4411.3 ~ 4485.7 cm-1及4307.1 ~ 4188.1 cm-1。当归产区根际土壤具有较为明显的地域性分布特征,根际土壤的NIRDRS指纹图谱与土壤类型密切相关。

关键词: 当归, 根际土壤, 土壤类型, 近红外漫反射光谱, 指纹图谱, 正交偏最小二乘-判别分析

Abstract: Near infrared diffuse reflectance spectrum (NIRDRS) fingerprints of 147 batches of Rhizosphere soil from 11 areas produced Angelica sinensis of Gansu Province were collected by integral sphere diffuse reflection,and the characteristic absorption peaks of NIRDRS fingerprints were analyzed.The similarity analysis of NIRDRS fingerprints were carried out by "correlation coefficient method",and orthogonal partial least squares-discriminant analysis (OPLS-DA) model was established to study the relationship between soil type and area division.The NIRDRS fingerprints,first derivative spectra and second derivative spectra of Rhizosphere soil in 8 main producing counties were similar,but three producing areas such as Lintao County,Lintan County and Kangle County were obviously different with other producing areas (especially Min County).The NIRDRS fingerprints of the Rhizosphere soils in each producing area can be clearly distinguished based on the OPLS-DA model,and main wave numbers range of the differences existed 7352.5 ~ 6935.9 cm-1,5819.9 ~ 5760.4 cm-1,4927.1 ~4609.7 cm-1,4411.3 ~ 4485.7 cm-1,and 4307.1 ~ 4188.1 cm-1.Rhizosphere soil from Angelica sinensis has a distinct regional distribution,and the NIRDRS fingerprints of Rhizosphere soil was closely related to soil types.

Key words: Angelica sinensis, Rhizosphere soil, soil type, near infrared diffuse reflectance spectrum, fingerprints, orthogonal partial least squares-discriminant analysis

中图分类号: 

R282.2 S151.9