HPLC-CAD测定复方氨基丁酸维E胶囊中GABA的含量

天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (增刊1): 124-128.

HPLC-CAD测定复方氨基丁酸维E胶囊中GABA的含量

陈红英1，林耿雪2，王健松1，袁晓2,3*

1广州白云山医药集团股份有限公司白云山制药总厂，广州 510515；2广州牌牌生物科技有限公司，广州 510540；3中国科学院武汉植物园，武汉 430074

出版日期:2018-07-31 发布日期:2018-07-31

Determination of GABA Content in Compound Aminobutyric Acid and Vitamin E Capsule by HPLC-CAD

CHEN Hong-ying 1, LIN Geng-xue 2, WANG Jian-song1, YUAN Xiao2,3*

1Guangzhou Bai Yun Shan pharmaceutical group co.,LTD.,Bai Yun Shan Pharmaceutical Factory,Guangzhou，510515,China; 2Guangzhou PI & PI Biotech Inc.Ltd.,Guangzhou,510540,China; 3Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan,430074,China

Online:2018-07-31 Published:2018-07-31

摘要/Abstract

摘要： 采用高效液相联合CAD检测器，Hypersil GOLD C₁₈色谱柱，以0.05%九氟戌酸水：甲醇（97∶3）为流动相等度洗脱，根据γ-氨基丁酸（GABA）对照品标准曲线及方程测定复方氨基丁酸维E胶囊中GABA的含量。本方法的线性范围为52~415 μg*mL^-1，线性方程为y=-0.00004x²+0.0602x+1.6924,r=0.9997，平均加样回收率为101.6%，RSD%为1.9%（n=9）。本法测定简便，快速，可用于复方氨基丁酸维E胶囊中GABA含量的测定。

关键词: 复方氨基维E胶囊；&gamma, -氨基丁酸；CAD检测器；高效液相色谱法

Abstract: This experiment was done by using HPLC combined with CAD.Hypersil GOLD C₁₈was used as the chromatographic column.The mixture solution of Nonafluoropentanoic acid (0.05% in water) and methanol with a proportion of 97∶3 was used as the mobile phase for the isocratic elution.Determination of GABA content in compound aminobutyric acid and vitamin E capsule was done by comparison of the described curve and equation of the standard GABA compound.The Linear equation in this experiment was described as y=-0.00004x²+0.0602x+1.6924,r=0.9997,with the linear range of 52~415 μg*mL^-1.The mean recovery of samples was 101.6% and RSD% was 1.9% (n=9).This method is simple and convenient,which could give a rapid determination of GABA content in aminobutyric acid vitamin E capsule.

Key words: aminobutyric acid vitamin E capsule, GABA, CAD, HPLC

中图分类号: O656.3 R917

陈红英1, 林耿雪2, 王健松1, 袁晓2, 3. HPLC-CAD测定复方氨基丁酸维E胶囊中GABA的含量[J]. 天然产物研究与开发, 2018, 30(增刊1): 124-128.

CHEN Hong-ying 1, LIN Geng-xue 2, WANG Jian-song1, YUAN Xiao2, 3. Determination of GABA Content in Compound Aminobutyric Acid and Vitamin E Capsule by HPLC-CAD[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2018, 30(增刊1): 124-128.