天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (增刊1): 65-71.

• 研究论文 • 上一篇    下一篇

不同枸杞多糖提取方法工艺优化与对比研究

田晓静1,刘元林1,吴启康1,龙鸣1,俸江宁1,陈士恩1,李明生1,2*,高丹丹1,马忠仁1,2   

  1. 1西北民族大学生命科学与工程学院,兰州 730124;2甘肃省动物细胞工程技术研究中心,兰州 730030
  • 出版日期:2018-07-31 发布日期:2018-07-31
  • 基金资助:

    国家自然科学基金(31560477);甘肃省科技计划(17YF 1WA166);西北民族大学“双一流”引导专项生物工程特色学科(10018703,1001070204)

     

Optimization and Comparison of Extraction Methods for Lycium barbarum Polysaccharides  

TIAN Xiao-jing1, LIU Yuan-lin1, WU Qi-kang1, LONG Ming1, FENG Jiang-ning1, CHEN  Shi-en1, LI Ming-sheng1,2*, GAO Dan-dan1, MA Zhong-ren1,2   

  1. 1College of life science and Engineering,Northwest Minzu University,Lanzhou 730124,China; 2Engineering Technology Research Center for Animal Cell,GanSu,Lanzhou 730030,China
  • Online:2018-07-31 Published:2018-07-31

摘要: 枸杞多糖是枸杞中主要活性成分之一,也是评价枸杞品质的重要指标之一。为有效对比不同枸杞多糖提取方法的差异,本研究采用单因素和正交试验优化浸提法、超声波辅助法和酶解法提取枸杞多糖的工艺,结果发现较佳条件分别如下,溶剂浸提法:温度90 ℃,时间3 h,料液比1∶30 g/mL;超声波辅助法:提取温度60 ℃,提取时间50 min,料液比1∶25 g/mL,pH为8;酶解法:酶解温度50 ℃,酶解时间160 min,加酶量0.50%,pH为5.0。在此基础上,提取宁夏中宁枸杞子中多糖,发现酶解法提取率最高(7.79%),其次为超声波辅助提取法(5.92%),浸提法的提取率最低(4.67%),为进一步开发和利用枸杞多糖的提供方法基础。

关键词: 枸杞多糖, 提取方法, 优化

Abstract: As one of the most important actice components in Lycium barbarum fruits,Lycium barbarum polysaccharide (LBP) is an important indexes to evaluate the quality of Lycium barbarum fruits.For comparison of the effects of different extraction methods for LBP,single factor and orthogonal test were employed to optimize the extraction process for methods of aqueous extraction,ultrasonic assisted extraction and enzyme assisted extraction.The results showed that the better condition is as follows,for aqueous extraction:temperature 90 ℃,time 3 h,Solid-liquid ratio 1:30 g/mL,for ultrasonic assisted extraction:extraction temperature 60 ℃,extracting time 50 min,Solid-liquid ratio of 1:25 g/mL,pH of 8,and for enzyme assisted extraction:enzymatic hydrolysis temperature 50 ℃,160 min of enzymolysis time with enzyme content of 0.50%,pH of 5.0.Applied to extract the LBP in Lycium barbarum fruits produced in Zhongning,Ningxia province,it was found that the enzyme hydrolysis extraction yield the highest (7.79%),followed by ultrasonic assisted extraction (5.92%),then extraction rate of aqueous extraction of the lowest (4.67%),laid solid foundation for further development and utilization of LBP.

Key words: Lyciumbar barum polysaccharide (LBP), extraction method, optimization  

中图分类号:  TS202.1 R284.2