天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (增刊2): 108-113.

• 研究简报 • 上一篇    下一篇

超微粉碎对金钗石斛的结构和抗氧化活性的影响研究

吴振1,李红2*,陈岗1,罗 杨1,詹永1*,杨勇1,3,谭红军1,黄小平1   

  1. 1重庆市中药研究院 中药健康学重庆市重点实验室,重庆 400065;2重庆市食品药品检验检测研究院,重庆 401123;3重庆市中药大健康工程技术研究中心,重庆 400065
  • 出版日期:2019-01-18 发布日期:2019-01-18
  • 基金资助:

    重庆市应用开发计划(重点项目)(cstc2014yykfB100 12);重庆市集成示范计划(cstc2015jcsf-kjfp80014);重庆市重点产业共性关键技术创新专项(csct2017zdcy -zdyfx0013)

Effect of Ultramicro Pulverization on Structure and Antioxidant Activities of Dendrobium Nobile

WU Zhen1,LI Hong2*,CHEN Gang1,LUO Yang1,ZHAN Yong1*,YANG Yong1,3,TAN Hong-jun1,HUANG Xiao-ping1   

  1. 1Chongqing Academy of Chinese Materia Medica,Chongqing Key Laboratory of Chinese Medicine & Health Science,Chongqing 400065,China; 2Chongqing Institute for Food and Drug Control,Chongqing 401121,China; 3Chongqing Engineering Technology Research Center of Materia Medica Health,Chongqing 402460,China
  • Online:2019-01-18 Published:2019-01-18

摘要: 研究了金钗石斛经超微粉碎和普通粉碎后其颗粒结构和抗氧化能力。研究发现,超微粉碎导致了金钗石斛药材大分子成分结构的改变,对细胞壁网状结构及粗纤维等成分具有破坏作用;同时其粒径较小、且分布集中。进一步采用体外还原力、清除DPPH·、OH·和O2–·等方法综合评价了金钗石斛超微粉和普通粗粉的抗氧化能力,与普通粉碎相比,超微处理使金钗石斛提取液的还原力、清除DPPH·、OH·和O2–·能力分别提高了1.6、1.4、1.5、1.5倍(金钗石斛提取液浓度为60 mg/mL时)。超微粉碎技术改变了金钗石斛的物理化学特性,破坏了其原有规则结构,提高了抗氧化能力,在医药和食品工业应用方面具有一定的潜力。

关键词: 金钗石斛, 超微粉碎, 普通粉碎, 结构, 扫描电镜, 抗氧化活性

Abstract: In order to study the application value of Dendrobium nobile,we investigated the particle structure and antioxidant activities of ultramicro powder and common powder of Dendrobium nobile.It was found that the ultrastructure of Dendrobium nobile was changed by ultramicro pulverization,and the cell wall net-like structure and fiber components were destroyed.The particle size was small and its distribution was more concentrated.Furthermore,the reduce power,and scavenging abilities of DPPH·,OH· and O2–· were studied.The reduce power,and scavenging abilities of DPPH·,OH· and O2–· has been increased by 1.6,1.4,1.5,1.5 times,respectively,compared to those extracted solution (The concentration of extracted solution:60 mg/mL).Ultramicro pulverization has changed the physical-chemical properties of Dendrobium nobile,destroyed its original structure,improved its antioxidant capacities,and had a certain potential in the application of medicine and food industries.

Key words: Dendrobium nobile, submicron powder, common powder, structure, SEM, antioxidant activity

中图分类号:  R282.4 Q94