UPLC-QTOF/MS法研究甜菊糖结晶过程中化学成分的分布

天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (增刊2): 114-120.

UPLC-QTOF/MS法研究甜菊糖结晶过程中化学成分的分布

额尔敦巴雅尔1,2 *，王欢欢1,2，焦利卫1,2，赵鹏宇1,2，杨文江1,2

1河北省天然色素工程技术研究中心；2晨光生物科技集团股份有限公司，邯郸 057250

出版日期:2019-01-18 发布日期:2019-01-18

Distribution of Each Component in Stevia of Crystallization Process by UPLC-QTOF/MS

Eerdunbayaer1,2*,WANG Huan-huan1,2,JIAO Li-wei1,2,ZHAO Peng-yu1,2,YANG Wen-jiang1,2

1Hebei Industrial Technology Institute of Plant Natural Pigments;2Chenguang Biotech Group Limited Corporation,Handan 057250,China

Online:2019-01-18 Published:2019-01-18

摘要/Abstract

摘要： 本研究通过超高效液相-串联四级杆飞行时间质谱（Ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry，UPLC-QTOF/MS），对甜菊糖原糖和结晶糖的成分进行了分析鉴定：结果显示：甜菊糖原糖中共鉴定45种物质，甜菊糖苷类17种，氨基酸类21种，黄酮类6种，皂苷类1种；而结晶糖中共鉴定出9种物质，含甜菊糖苷类8种，氨基酸类1种，此研究明确了甜菊糖结晶过程中各物质去向及分布情况，为提高RA产品含量提供了理论依据。

关键词: 甜菊糖, 超高效液相-串联四级杆飞行时间质谱, 结晶, 化学成分摘

Abstract: In this study,the composition of stevia and crystallization of stevia has been studie dy ultra high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS).The results showed that 45 kinds of substances were identified in stevia,among which,there were 17 species of stevia glycoside,21 amino acids,6 kinds of flavonoids,and 1 kind of saponin.9 kinds of substances were identified in the crystallization sugar,8 species of stevia glycoside and 1 kinds of amino acids.The whereabouts and distribution of various substances in the process of crystallization of stevia were clarified.A theoretical basis for improving the content of RA products was provided.

Key words: stevioside, ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS), crystallization, chemical compositio

中图分类号: R284.1 Q946.91

额尔敦巴雅尔1, 2 , 王欢欢1, 2, 焦利卫1, 2, 赵鹏宇1, 2, 杨文江1, 2. UPLC-QTOF/MS法研究甜菊糖结晶过程中化学成分的分布[J]. 天然产物研究与开发, 2018, 30(增刊2): 114-120.

Eerdunbayaer1, 2, WANG Huan-huan1, 2, JIAO Li-wei1, 2, ZHAO Peng-yu1, 2, YANG Wen-jiang1, 2. Distribution of Each Component in Stevia of Crystallization Process by UPLC-QTOF/MS[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2018, 30(增刊2): 114-120.

[1]	杨文江1, 2, 赵鹏宇1, 2, 焦利卫1, 2, 王欢欢1, 2, 额尔敦巴雅尔1, 2. 利用UPLC-QTOF-MS研究番茄籽成分[J]. 天然产物研究与开发, 2018, 30(增刊1): 104-106.
[2]	伍英，张桂梅，岩利，姜士宽*. 天然橡胶乳清中白坚木皮醇的规模化提取技术研究[J]. 天然产物研究与开发, 2017, 29(6): 1035-1038.
[3]	陈媛，唐铁鑫，陈金龙，蒋林. 高效率高纯度绿原酸制备工艺研究[J]. 天然产物研究与开发, 2014, 26(9): 1503-1509.
[4]	胡林1，寇彦杰2*，翁永京1，万小玲1. 高效液相色谱法测定甜菊糖苷中甜菊醇的含量[J]. 天然产物研究与开发, 2014, 26(10): 1644-1646.
[5]	邓乾春，黄凤洪，黄庆德，张品，李江涛. α-亚麻酸甾醇酯的理化特性及氧化稳定性研究[J]. 天然产物研究与开发, 2012, 23(3): 370-373.