天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (增刊2): 163-166.

• 研究简报 • 上一篇    下一篇

5种石斛抗衰老与美白活性研究

袁明焱1,3,王雅琴1, 李一泽1,谢勇1,杨柳1,2*   

  1. 1中国科学院昆明植物研究所植物医生研发中心;2中国科学院昆明植物研究所植物化学与西部资源持续利用国家重点实验室,昆明 650201;3安徽中医药大学药学院,合肥 230011
  • 出版日期:2019-01-18 发布日期:2019-01-18
  • 基金资助:

    石斛抗衰老、美白及石斛花芳香成分研究(Y65J8232 C1)

Study on Anti-aging and Whitening Activity of Five Dendrobium Species from “Comestic List”

YUAN Ming-yan1,3,WANG Ya-qin1,LI Yi-zhe1,XIE Yong1,YANG Liu1,2*   

  1. 1R & D Center of Dr.Plant,Kunming Institute of Botany,Chinese Academy of Sciences; 2State Key Laboratory of Phytochemistry and plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China; 3College of pharmacy,Anhui University of Chinese Medicine,Hefei 230011,China
  • Online:2019-01-18 Published:2019-01-18

摘要: 分别采用水与乙醇提取获得石斛茎的水提物与醇提物,选用DPPH自由基清除实验、酪氨酸酶活性抑制实验与HDFa胶原蛋白分泌实验来考察《化妆品名录》收载的5种石斛—铁皮石斛,金钗石斛,美花石斛,束花石斛,流苏石斛的抗衰老与美白活性。研究发现,铁皮石斛醇提物在100 μg/mL浓度下,DPPH自由基清除率为55.416%;金钗石斛水提物与铁皮石斛水提物在100 μg/mL浓度下,酪氨酸酶抑制率分别为8.023%与8.488%;束花石斛水提物在10 μg/mL浓度下,胶原蛋白分泌增加率为22.153%。结果表明,在100 μg/mL浓度下,铁皮石斛醇提物有较强的自由基清除活性,金钗石斛水提物与铁皮石斛水提物有微弱的酪氨酸酶抑制活性;束花石斛水提物在10 μg/mL浓度下,有较好的促进胶原蛋白分泌作用。

关键词: 石斛, 化妆品名录, 抗衰老, 美白活性

Abstract: There were five Dendrobium species:Dendrobium officinale,Dendrobium nobile,Dendrobium loddigesii,Dendrobium chrysanthum and Dendrobium fimbriatum in the "Cosmetics List".The stems of the five Dendrobium species were extracted by water and ethanol to obtain their aqueous extracts and alcoholic extracts,respectively.Then their anti-aging and whitening activities were investigated by DPPH radical scavenging experiment,tyrosinase activity inhibition test and HDFa collagen secretion test.At the concentration of 100 μg/mL,DPPH free radical scavenging rate of the ethanol extract of Dendrobium officinale was 55.416% and the tyrosinase inhibition rates of water extract of Dendrobium nobile and Dendrobium officinale were 8.023% and 8.488%,respectively;The increase rate of collagen secretion of water extract of Dendrobium chrysanthum was 22.153% at 10 μg/mL.The results indicated the ethanol extract of Dendrobium officinale had a strong free radical scavenging activity,and the water extracts of Dendrobium nobile and Dendrobium officinale had weak tyrosinase inhibitory activities at 100 μg/mL.The water extract of Dendrobium chrysanthum had a good role in promoting collagen secretion at 10 μg/mL.This study built the foundation for the development and utilization of Dendrobium in Cosmetics.

Key words: Dendrobium, Cosmetics List, anti-aging, whitening activity

中图分类号:  R961.1 R284.2